Aldehyde dehydrogenase 2/ALDH2 Double Nickase Plasmid (h): sc-400809-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Aldehyde dehydrogenase 2/ALDH2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Aldehyde dehydrogenase 2/ALDH2 Double Nickase Plasmid (h) and Aldehyde dehydrogenase 2/ALDH2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ALDH2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Aldehyde dehydrogenase 2/ALDH2 Antibody (3D12): sc-100496
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Aldehyde dehydrogenase 2/ALDH2 Double Nickase Plasmid (h) | sc-400809-NIC | 20 µg | $410.00 | |||
Aldehyde dehydrogenase 2/ALDH2 Double Nickase Plasmid (h2) | sc-400809-NIC-2 | 20 µg | $410.00 |
ALDH2 encodes the mitochondrial aldehyde dehydrogenase 2 enzyme, a key catalyst in NAD(P)+-dependent oxidation of reactive aldehydes generated from ethanol metabolism and lipid peroxidation. By clearing acetaldehyde and toxic lipid-derived aldehydes such as 4-hydroxynonenal, ALDH2 supports mitochondrial redox balance, limits oxidative stress, and protects proteins and membranes from aldehyde adduct formation. ALDH2 activity interfaces with mitochondrial quality control, ROS signaling, and broader xenobiotic and intermediary metabolism pathways. Altered ALDH2 function has been associated with differential susceptibility to aldehyde-induced cellular injury and is frequently studied in contexts including alcohol-related tissue stress, cardiometabolic phenotypes, and neurodegenerative disease mechanisms.
Aldehyde dehydrogenase 2/ALDH2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ALDH2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ALDH2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ALDH2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ALDH2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.