ALCAM Double Nickase Plasmid (m): sc-419076-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ALCAM Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ALCAM Double Nickase Plasmid (m) and ALCAM Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Alcam. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ALCAM Antibody (B-6): sc-74558
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ALCAM Double Nickase Plasmid (m) | sc-419076-NIC | 20 µg | $410.00 | |||
ALCAM Double Nickase Plasmid (m2) | sc-419076-NIC-2 | 20 µg | $410.00 |
Alcam encodes activated leukocyte cell adhesion molecule (ALCAM/CD166), an immunoglobulin superfamily adhesion receptor that supports homophilic and heterophilic interactions at the cell surface. In mouse tissues, ALCAM contributes to cell–cell contact stabilization, epithelial and endothelial organization, and guidance of migrating cells, linking membrane adhesion complexes to cytoskeletal remodeling programs. ALCAM-mediated adhesion intersects with processes such as immune cell trafficking, neurite outgrowth, and stem/progenitor niche maintenance, where altered adhesion dynamics can shift differentiation and tissue architecture. Dysregulated ALCAM expression or localization has been associated with changes in invasion and metastatic potential in cancer models and with inflammatory microenvironments, making Alcam a useful node for studying adhesion-dependent signaling and tissue remodeling.
ALCAM Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Alcam locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Alcam. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Alcam function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Alcam-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.