Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ALCAM Double Nickase Plasmid (h): sc-418418-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ALCAM Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ALCAM Double Nickase Plasmid (h) and ALCAM Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ALCAM. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ALCAM Antibody (B-6): sc-74558
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ALCAM Double Nickase Plasmid (h) | sc-418418-NIC | 20 µg | $410.00 | |||
ALCAM Double Nickase Plasmid (h2) | sc-418418-NIC-2 | 20 µg | $410.00 |
ALCAM (activated leukocyte cell adhesion molecule, CD166) is an immunoglobulin superfamily cell-surface glycoprotein that mediates homophilic ALCAM–ALCAM and heterophilic ALCAM–CD6 interactions to regulate cell–cell adhesion. It contributes to leukocyte trafficking and immune synapse organization, and it influences cell migration and tissue architecture through adhesion-dependent signaling and cytoskeletal remodeling. In human tissues, ALCAM expression is commonly studied in endothelial and epithelial compartments as well as immune cell subsets, where it can modulate inflammatory responses and barrier function. Dysregulated ALCAM has been associated with altered adhesion, invasion, and metastatic behavior in multiple tumor contexts, making it a useful entry point for investigating adhesion-driven phenotypes and microenvironmental interactions.
ALCAM Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ALCAM locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ALCAM. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ALCAM function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ALCAM-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.