Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
AKAP 12 Double Nickase Plasmid (h): sc-406008-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- AKAP 12 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- AKAP 12 Double Nickase Plasmid (h) and AKAP 12 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting AKAP12. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: AKAP 12 Antibody (C-12): sc-376740
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
AKAP 12 Double Nickase Plasmid (h) | sc-406008-NIC | 20 µg | $410.00 | |||
AKAP 12 Double Nickase Plasmid (h2) | sc-406008-NIC-2 | 20 µg | $410.00 |
AKAP12 encodes A-kinase anchoring protein 12 (AKAP 12), a scaffolding protein that spatially organizes PKA, PKC, and phosphatases to coordinate compartmentalized signaling at the plasma membrane and cytoskeleton. By integrating cues from GPCR- and growth factor–driven pathways, AKAP 12 influences cell shape, motility, adhesion dynamics, and cytoskeletal remodeling through regulation of actin-associated processes. Altered AKAP12 expression or signaling context has been linked to dysregulated migration and proliferation programs relevant to cancer biology and vascular/inflammatory responses. These properties make AKAP12 a useful target for studying how anchored kinase signaling governs barrier function, stress responses, and microenvironment-dependent phenotypes.
AKAP 12 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the AKAP12 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within AKAP12. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt AKAP12 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of AKAP12-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.