AK4 Double Nickase Plasmid (h): sc-416951-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- AK4 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- AK4 Double Nickase Plasmid (h) and AK4 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting AK4. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: AK4 Antibody (A-9): sc-271161
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
AK4 Double Nickase Plasmid (h) | sc-416951-NIC | 20 µg | $410.00 | |||
AK4 Double Nickase Plasmid (h2) | sc-416951-NIC-2 | 20 µg | $410.00 |
AK4 (adenylate kinase 4) is a mitochondrial nucleoside monophosphate kinase that helps balance adenine nucleotide pools and supports cellular energy homeostasis under metabolic stress. It contributes to mitochondrial function by influencing ATP/ADP/AMP interconversion and links to pathways regulating oxidative phosphorylation, redox control, and adaptive responses to hypoxia. Altered AK4 expression has been associated with changes in mitochondrial integrity and stress signaling programs that shape cell survival, proliferation, and motility. As a metabolic regulator, AK4 is frequently studied in contexts where mitochondrial rewiring and stress tolerance are central features of disease biology, including cancer and cardiometabolic dysfunction.
AK4 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the AK4 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within AK4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt AK4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of AK4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.