AK2 Double Nickase Plasmid (h): sc-404166-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- AK2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- AK2 Double Nickase Plasmid (h) and AK2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting AK2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: AK2 Antibody (F-2): sc-374095
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
AK2 Double Nickase Plasmid (h) | sc-404166-NIC | 20 µg | $410.00 | |||
AK2 Double Nickase Plasmid (h2) | sc-404166-NIC-2 | 20 µg | $410.00 |
Adenylate kinase 2 (AK2) is a mitochondrial intermembrane space phosphotransferase that catalyzes reversible phosphate transfer among adenine nucleotides, supporting ATP/ADP/AMP homeostasis and mitochondrial energy exchange. By buffering adenine nucleotide pools, AK2 contributes to oxidative phosphorylation-linked metabolism, apoptotic signaling dynamics, and nucleotide-dependent processes that influence cellular proliferation and stress responses. Disruption of AK2 function has been associated with impaired hematopoietic and immune cell development and mitochondrial dysfunction phenotypes, making it relevant to studies of metabolic regulation, apoptosis, and cell fate control. In human systems, AK2 is frequently investigated in the context of mitochondrial biology, bioenergetic stress, and mechanisms connecting energy balance to disease-associated cellular dysfunction.
AK2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the AK2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within AK2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt AK2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of AK2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.