AK1 Double Nickase Plasmid (h): sc-407286-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- AK1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- AK1 Double Nickase Plasmid (h) and AK1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting AK1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: AK1 Antibody (E-8): sc-365316
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
AK1 Double Nickase Plasmid (h) | sc-407286-NIC | 20 µg | $410.00 | |||
AK1 Double Nickase Plasmid (h2) | sc-407286-NIC-2 | 20 µg | $410.00 |
Adenylate kinase 1 (AK1) is a cytosolic phosphotransferase that maintains adenine nucleotide homeostasis by catalyzing the reversible conversion of ATP and AMP to two molecules of ADP, supporting rapid energy buffering in cells with fluctuating demands. By shaping local ATP/ADP/AMP ratios, AK1 influences metabolic control points connected to glycolysis, oxidative phosphorylation, and AMP-sensitive signaling, including crosstalk with cellular energy-stress responses. AK1 activity is particularly relevant in tissues with high energetic flux such as muscle and erythrocytes, where perturbations in nucleotide balance can affect contractility and red cell stability. Variation in AK1 expression or function has been examined in the context of metabolic phenotypes and disorders linked to energy dysregulation, providing a mechanistic entry point for studying nucleotide metabolism in human cells.
AK1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the AK1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within AK1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt AK1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of AK1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.