Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Aiolos CRISPR/Cas9 KO Plasmid (h): sc-400764
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Aiolos CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Aiolos genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Aiolos Antibody (3H5-G7): sc-293421
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Aiolos CRISPR/Cas9 KO Plasmid (h) | sc-400764 | 20 µg | $397.00 |
Overview
IKZF3 encodes Aiolos, a zinc finger transcription factor of the Ikaros family that regulates lymphocyte differentiation and immune homeostasis by controlling lineage-specific gene expression programs. Aiolos modulates chromatin accessibility and transcriptional repression/activation networks that shape B cell maturation, plasma cell development, and T cell functional states, integrating signals downstream of antigen receptor pathways. Altered IKZF3 activity has been associated with dysregulated immune responses and perturbed lymphoid cell fate decisions, making it relevant to studies of autoimmunity, inflammation, and hematologic malignancy biology. In human cells, Aiolos-dependent transcriptional circuitry influences cytokine signaling outputs and checkpointing of activation versus differentiation programs.
Aiolos CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the IKZF3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the IKZF3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the IKZF3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Aiolos protein expression.
This CRISPR knockout system enables efficient generation of IKZF3-deficient cell models for investigation of Aiolos signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting IKZF3 exon(s) critical for Aiolos function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple IKZF3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Aiolos CRISPR/Cas9 KO Plasmid (h) and Aiolos CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the IKZF3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Aiolos HDR Plasmid (h) and Aiolos HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by IKZF3 homology arms to support homology-directed repair at defined IKZF3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.