AHNAK Antibody (E-5): sc-390743

AHNAK Antibody (E-5) is a mouse monoclonal IgG2a kappa light chain antibody that detects AHNAK protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). AHNAK (E-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. AHNAK protein, also known as neuroblast differentiation-associated protein AHNAK or desmoyokin, is a large 5,890 amino acid protein encoded by the intronless human gene AHNAK located on chromosome 11q12. AHNAK features a complex structure with three main regions: a 251 amino acid N-terminus, a substantial central region comprising 4,390 amino acids with multiple repeated units, and a 1,002 amino acid C-terminus. The central region is characterized by antiparallel beta-strands linked by loops, which is crucial for structural integrity and function. The C-terminal region contains several putative regulatory elements, including nuclear export localization signals, a leucine zipper, and potential phosphorylation sites for key kinases such as Akt1 and protein kinase C. These features suggest AHNAK plays a significant role as a signaling molecule in various physiological processes, including neuronal cell differentiation and modulation of cardiac L-type calcium channel function in response to β-adrenergic stimulation.

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AHNAK Antibody (E-5) References:

1. Spinal cord injury-induced up-regulation of AHNAK, expressed in cells delineating cystic cavities, and associated with neoangiogenesis.  |  von Boxberg, Y., et al. 2006. Eur J Neurosci. 24: 1031-41. PMID: 16930430
2. Identification of an AHNAK binding motif specific for the Annexin2/S100A10 tetramer.  |  De Seranno, S., et al. 2006. J Biol Chem. 281: 35030-8. PMID: 16984913
3. Ahnak, a new player in beta-adrenergic regulation of the cardiac L-type Ca2+ channel.  |  Haase, H. 2007. Cardiovasc Res. 73: 19-25. PMID: 17045254
4. AHNAK, a novel component of the dysferlin protein complex, redistributes to the cytoplasm with dysferlin during skeletal muscle regeneration.  |  Huang, Y., et al. 2007. FASEB J. 21: 732-42. PMID: 17185750
5. [Effect of hypoxia on the gene profile of human bone marrow-derived mesenchymal stem cells].  |  Wu, EH., et al. 2007. Sheng Li Xue Bao. 59: 227-32. PMID: 17437048
6. Enlargeosome traffic: exocytosis triggered by various signals is followed by endocytosis, membrane shedding or both.  |  Cocucci, E., et al. 2007. Traffic. 8: 742-57. PMID: 17488290
7. Ahnak protein activates protein kinase C (PKC) through dissociation of the PKC-protein phosphatase 2A complex.  |  Lee, IH., et al. 2008. J Biol Chem. 283: 6312-20. PMID: 18174170
8. Ahnak functions as a tumor suppressor via modulation of TGFβ/Smad signaling pathway.  |  Lee, IH., et al. 2014. Oncogene. 33: 4675-84. PMID: 24662814
9. Regulation of c-Myc Expression by Ahnak Promotes Induced Pluripotent Stem Cell Generation.  |  Lim, HJ., et al. 2016. J Biol Chem. 291: 752-61. PMID: 26598518
10. AHNAK as a Prognosis Factor Suppresses the Tumor Progression in Glioma.  |  Zhao, Z., et al. 2017. J Cancer. 8: 2924-2932. PMID: 28928883
11. Ahnak deficiency attenuates high-fat diet-induced fatty liver in mice through FGF21 induction.  |  Kim, YN., et al. 2021. Exp Mol Med. 53: 468-482. PMID: 33785868
12. AHNAK, regulated by the OSM/OSMR signaling, involved in the development of primary localized cutaneous amyloidosis.  |  Liu, H., et al. 2023. J Dermatol Sci. 110: 53-60. PMID: 37100691

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-AHNAK Antibody (E-5) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-AHNAK Antibody (E-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

AHNAK Antibody (E-5) Specifications

Alternate Product Name: AHNAK

Product Clone Name: E-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 5568-5720

Epitope Target: AHNAK

Epitope Species: human

Product Reactivity/Detection: AHNAK

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for AHNAK Antibody (E-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin