AGS3 Double Nickase Plasmid (h): sc-402802-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- AGS3 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- AGS3 Double Nickase Plasmid (h) and AGS3 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GPSM1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: AGS3 Antibody (G-2): sc-271721
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
AGS3 Double Nickase Plasmid (h) | sc-402802-NIC | 20 µg | $410.00 | |||
AGS3 Double Nickase Plasmid (h2) | sc-402802-NIC-2 | 20 µg | $410.00 |
GPSM1 encodes activator of G-protein signaling 3 (AGS3), a multidomain regulator that binds Gαi/o subunits and modulates heterotrimeric G-protein cycling independently of classical GPCR activation. Through its TPR and GPR/GoLoco motifs, AGS3 scaffolds signaling complexes that influence receptor desensitization, vesicle trafficking, and polarity-associated processes, integrating inputs from cAMP/PKA and small GTPase networks. AGS3 activity has been linked to control of cell migration and differentiation programs, and altered GPSM1 expression or signaling balance has been reported in contexts of neurobiology and tumor-associated signaling rewiring. These properties make GPSM1 a useful target for dissecting G-protein–regulated pathway crosstalk and identifying downstream effectors that shape cellular state.
AGS3 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GPSM1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GPSM1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GPSM1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GPSM1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.