AFP Receptor Antibody (5E1): sc-51752

AFP Receptor Antibody (5E1) is a mouse monoclonal IgG2a antibody that detects the AFP Receptor protein of human origin by western blotting (WB), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-AFP Receptor antibody (5E1) specifically targets the alpha-fetoprotein receptor (AFP Receptor or AFP-R), a crucial protein located on the cell surface of placental villous tissues, where AFP-R plays a vital role in mediating the uptake of alpha-fetoprotein (AFP) by proliferating or differentiating cells. AFP Receptor (5E1) antibody recognizes a protein significant in fetal development, as AFP is produced in varying levels during this period and is typically found only in trace amounts in normal adult tissues. Notably, AFP can be detected at elevated levels in hepatocellular carcinomas and in the plasma and ascitic fluid of adults with hepatoma, making AFP Receptor a potential tumor marker. Furthermore, high expression levels of AFP Receptor have been observed in gastric cancer cells, correlating with tumor cell growth. The regulation of AFP expression involves transcription factors such as hepatocyte nuclear factor-1 (HNF-1), nuclear factor-1 (NF-1), and C/EBP, which interact with the AFP promoter to modulate activity. HNF-1 enhances AFP expression, while NF-1 binding leads to a reduction in promoter activity, highlighting the intricate control of AFP Receptor in cancer biology.

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AFP Receptor Antibody (5E1) References:

1. Alpha-fetoprotein enhances the proliferation of human hepatoma cells in vitro.  |  Wang, XW. and Xie, H. 1999. Life Sci. 64: 17-23. PMID: 10027738
2. Activation of an alpha-fetoprotein (AFP)/receptor autocrine loop in HT-29 human colon carcinoma cells.  |  Esteban, C., et al. 1991. Int J Cancer. 49: 425-30. PMID: 1717385
3. Increased alpha-fetoprotein receptor in the serum of patients with early-stage breast cancer.  |  Moro, R., et al. 2012. Curr Oncol. 19: e1-8. PMID: 22328843
4. A new system for targeted delivery of doxorubicin into tumor cells.  |  Yabbarov, NG., et al. 2013. J Control Release. 168: 135-41. PMID: 23517785
5. The receptor binding fragment of alpha-fetoprotein is a promising new vector for the selective delivery of antineoplastic agents.  |  Posypanova, GA., et al. 2013. J Drug Target. 21: 458-65. PMID: 23600748
6. The adenocarcinoma cell surface mucin receptor for alpha-fetoprotein: is the same receptor present on circulating monocytes and macrophages? A commentary.  |  Mizejewski, GJ. 2014. Tumour Biol. 35: 7397-402. PMID: 24916573
7. Recombinant human alpha fetoprotein synergistically potentiates the anti-cancer effects of 1'-S-1'-acetoxychavicol acetate when used as a complex against human tumours harbouring AFP-receptors.  |  Arshad, NM., et al. 2015. Oncotarget. 6: 16151-67. PMID: 26158863
8. A new paradigm about HERV-K102 particle production and blocked release to explain cortisol mediated immunosenescence and age-associated risk of chronic disease.  |  Laderoute, MP. 2015. Discov Med. 20: 379-91. PMID: 26760982
9. Recombinant alpha-fetoprotein receptor-binding domain co-expression with polyglutamate tags facilitates in vivo folding in E. coli.  |  Mollaev, M., et al. 2018. Protein Expr Purif. 143: 77-82. PMID: 29127003
10. Selective targeting of myeloid-derived suppressor cells in cancer patients through AFP-binding receptors.  |  Pak, VN. 2019. Future Sci OA. 5: FSO321. PMID: 30652015
11. High anti-tumor activity of a novel alpha-fetoprotein-maytansinoid conjugate targeting alpha-fetoprotein receptors in colorectal cancer xenograft model.  |  Griffin, P., et al. 2023. Cancer Cell Int. 23: 60. PMID: 37016369
12. Activation of an alpha-fetoprotein/receptor pathway in human normal and malignant peripheral blood mononuclear cells.  |  Esteban, C., et al. 1993. Leukemia. 7: 1807-16. PMID: 7694005

The preferred AFP Receptor antibody is AFP Receptor Antibody (2B8): sc-51751.