AFP/Alpha fetoprotein Antibody (AFP-11): sc-51506

AFP Antibody (AFP-11) is a mouse monoclonal IgG1 antibody engineered to detect Alpha-fetoprotein (AFP), also known as Alpha 1 Fetoprotein or Alpha-fetoglobulin, with exceptional affinity for the purified human AFP protein. Anti-AFP antibody (AFP-11) is recommended for use in western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP), ensuring precise detection of human AFP in various experimental settings. AFP is a vital glycoprotein predominantly synthesized in the fetal liver, where AFP plays a crucial role in transporting essential ligands such as copper, nickel, and fatty acids within the cell and extracellular environment. In adult tissues, AFP is typically present only in trace amounts; however, AFP expression is significantly elevated in hepatocellular carcinoma and other liver-related cancers, making AFP a critical biomarker for early cancer detection and monitoring tumor progression. The cellular localization of AFP, primarily within the cytoplasm and secreted into the extracellular matrix, is important for AFP function in ligand transport and involvement in cancer cell growth and metastasis. Additionally, the regulation of AFP expression is controlled by key transcription factors including HNF-1, which promotes AFP expression, and NF-1, which inhibits AFP promoter activity. AFP monoclonal antibody (AFP-11) provides researchers with a reliable tool to investigate AFP expression patterns, cellular distribution, and functional interactions, thereby advancing studies in developmental biology, oncology, and protein interaction research.

The preferred AFP/Alpha fetoprotein antibody is AFP/Alpha fetoprotein Antibody (C3): sc-8399.