AFP/Alpha fetoprotein Antibody (1B10): sc-80464

AFP Antibody (1B10) is a mouse monoclonal IgG1 kappa light chain antibody developed to detect Alpha-fetoprotein (AFP), also known as Alpha 1 Fetoprotein or Alpha-fetoglobulin, with exceptional affinity for AFP isolated from human amniotic fluid. Anti-AFP antibody (1B10) is validated for use in western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA), ensuring precise detection of human AFP in various experimental settings. AFP is a crucial glycoprotein predominantly synthesized in the fetal liver, where its structure includes extensive glycosylation, a vital post-translational modification that enhances stability, ligand-binding capacity, and transport functions. These modifications are essential for AFP′s ability to bind metals such as copper and nickel, as well as fatty acids, and to interact with serum albumin and estrogen, which are important for maintaining physiological balance during development. In adult tissues, AFP is typically present only in trace amounts; however, expression is significantly elevated in hepatocellular carcinoma and other liver-related cancers, making AFP a critical biomarker for early cancer detection and monitoring tumor progression. The post-translational modifications of AFP also influence immunogenicity and detection, highlighting the importance of using AFP monoclonal antibody (1B10) for reliable measurements. Additionally, AFP expression is regulated by key transcription factors including HNF-1, which promotes expression, and NF-1, which inhibits promoter activity. Anti-AFP antibody (1B10) provides researchers with a reliable tool to investigate AFP′s expression levels, post-translational modifications, and functional roles, thereby advancing studies in developmental biology, oncology, and protein interaction research.

The preferred AFP/Alpha fetoprotein antibody is AFP/Alpha fetoprotein Antibody (C3): sc-8399.