ADSL Antibody (C-11): sc-365623
- ADSL Antibody (C-11) is a mouse monoclonal IgG1 κ ADSL antibody, cited in 7 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 327-351 within an internal region of ADSL of human origin
- ADSL Antibody (C-11) is recommended for detection of ADSL of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-ADSL Antibody (C-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for ADSL Antibody (C-11) for WB applications. This reagent is now offered in a bundle with ADSL Antibody (C-11) (see ordering information below).
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SEE ALSO...
ADSL Antibody (C-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects ADSL protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ADSL (C-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ADSL protein, also known as adenylosuccinate lyase, AMPS, ASL, or ASASE, is a crucial 484 amino acid enzyme that plays a significant role in purine biosynthesis and the conversion of inosine monophosphate to adenosine monophosphate (AMP). ADSL is predominantly located in the cytoplasm, where ADSL catalyzes two essential reactions in AMP biosynthesis: the removal of fumarate from succinylaminoimidazole carboxamide ribotide (SAICA ribotide) to produce aminoimidazole carboxamide ribotide (AICA) and the subsequent removal of fumarate from adenylosuccinate to yield AMP. Proper functioning of ADSL is vital for maintaining cellular energy levels and nucleotide synthesis, and defects in the gene encoding ADSL can lead to adenylosuccinase deficiency, an autosomal recessive disorder associated with severe neurological symptoms, including epilepsy, growth retardation, and muscular wasting. Multiple isoforms of ADSL arise from alternative splicing, which may contribute to ADSL′s functional diversity and regulatory mechanisms within different tissues.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ADSL Antibody (C-11) References:
- Human adenylosuccinate lyase (ADSL), cloning and characterization of full-length cDNA and its isoform, gene structure and molecular basis for ADSL deficiency in six patients. | Kmoch, S., et al. 2000. Hum Mol Genet. 9: 1501-13. PMID: 10888601
- Clinical, biochemical and molecular genetic correlations in adenylosuccinate lyase deficiency. | Race, V., et al. 2000. Hum Mol Genet. 9: 2159-65. PMID: 10958654
- Determination, activity and biological role of adenylosuccinate lyase in blood cells. | Tabucchi, A., et al. 2001. Biomed Pharmacother. 55: 277-83. PMID: 11428554
- Mutation of a nuclear respiratory factor 2 binding site in the 5' untranslated region of the ADSL gene in three patients with adenylosuccinate lyase deficiency. | Marie, S., et al. 2002. Am J Hum Genet. 71: 14-21. PMID: 12016589
- Intrafamilial variability in the phenotypic expression of adenylosuccinate lyase deficiency: a report on three patients. | Edery, P., et al. 2003. Am J Med Genet A. 120A: 185-90. PMID: 12833398
- Two novel mutant human adenylosuccinate lyases (ASLs) associated with autism and characterization of the equivalent mutant Bacillus subtilis ASL. | Sivendran, S., et al. 2004. J Biol Chem. 279: 53789-97. PMID: 15471876
- Expression, purification, and characterization of stable, recombinant human adenylosuccinate lyase. | Lee, P. and Colman, RF. 2007. Protein Expr Purif. 51: 227-34. PMID: 16973378
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ADSL Antibody (C-11) | sc-365623 | 200 µg/ml | $316.00 | |||
ADSL Antibody (C-11): m-IgG Fc BP-HRP Bundle | sc-552012 | 200 µg Ab; 10 µg BP | $354.00 | |||
ADSL Antibody (C-11) AC | sc-365623 AC | 500 µg/ml, 25% agarose | $416.00 | |||
ADSL Antibody (C-11) HRP | sc-365623 HRP | 200 µg/ml | $316.00 | |||
ADSL Antibody (C-11) FITC | sc-365623 FITC | 200 µg/ml | $330.00 | |||
ADSL Antibody (C-11) PE | sc-365623 PE | 200 µg/ml | $343.00 | |||
ADSL Antibody (C-11) Alexa Fluor® 488 | sc-365623 AF488 | 200 µg/ml | $357.00 | |||
ADSL Antibody (C-11) Alexa Fluor® 546 | sc-365623 AF546 | 200 µg/ml | $357.00 | |||
ADSL Antibody (C-11) Alexa Fluor® 594 | sc-365623 AF594 | 200 µg/ml | $357.00 | |||
ADSL Antibody (C-11) Alexa Fluor® 647 | sc-365623 AF647 | 200 µg/ml | $357.00 | |||
ADSL Antibody (C-11) Alexa Fluor® 680 | sc-365623 AF680 | 200 µg/ml | $357.00 | |||
ADSL Antibody (C-11) Alexa Fluor® 790 | sc-365623 AF790 | 200 µg/ml | $357.00 | |||
ADSL (C-11) Neutralizing Peptide | sc-365623 P | 100 µg/0.5 ml | $68.00 |