AdipoR1 (h): 293T Lysate: sc-111211

AdipoR1 (h): 293T Lysate is derived from the 293T cell line, a human embryonic kidney cell line engineered to express the SV40 large T antigen, which enhances its protein production capabilities. This lysate is specifically enriched with human Adiponectin Receptor 1 (AdipoR1), a receptor critical for mediating the metabolic effects of adiponectin, a hormone involved in regulating glucose levels and fatty acid breakdown. AdipoR1 is known to activate AMP-activated protein kinase (AMPK) and peroxisome proliferator-activated receptor alpha (PPAR-α) pathways, which play significant roles in cellular energy homeostasis and lipid metabolism. In research, AdipoR1 (h): 293T Lysate is utilized to study the signaling mechanisms and metabolic functions mediated by AdipoR1. Researchers use this lysate in Western blotting to detect and quantify AdipoR1 expression under various experimental conditions, aiding in the understanding of receptor regulation and its interaction with signaling molecules. The lysate is also employed in co-immunoprecipitation assays to identify proteins that interact with AdipoR1, providing insights into the receptor's role in cellular signaling networks. Additionally, functional assays such as ligand binding and receptor activation studies are conducted using this lysate to explore how adiponectin binding influences AdipoR1-mediated signaling pathways. This research helps explain the molecular mechanisms through which AdipoR1 regulates metabolic processes. The use of AdipoR1 (h): 293T Lysate allows for a detailed investigation of receptor function and its involvement in metabolic regulation, contributing valuable insights into fundamental biological processes. The origin and quality of the 293T cell line are rigorously maintained to ensure reliable and reproducible results in experimental research.