Molecular structure of ADHP, CAS Number: 119171-73-2
ADHP (CAS 119171-73-2)
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Alternate Names:
10-Acetyl-3,7-dihydroxyphenoxazine; A 6550
Application:
ADHP is a fluorogenic substrate and stable hydrogen peroxide probe
CAS Number:
119171-73-2
Purity:
≥95%
Molecular Weight:
257.24
Molecular Formula:
C14H11NO4
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.
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SDS & Certificate of Analysis
ADHP is a non-fluorescent and highly sensitive and stable hydrogen peroxide probe. Experimentally used as a peroxidase fluorogenic substrate in the presence of HRP, a 1:1 stoichiometric reaction with hydrogen peroxide occurs, generating highly fluorescent resorufin. With absorption and fluorescence emission maxima at around 563 nm and 587 nm respectively, ADHP offers versatility in assay methods. Its high extinction coefficient of 54,000 cm-1M-1 enables the assay to be conducted using either fluorometric or spectrophotometric techniques.
ADHP (CAS 119171-73-2) References
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- Ordered cleavage of myeloperoxidase ester bonds releases active site heme leading to inactivation of myeloperoxidase by benzoic acid hydrazide analogs. | Huang, J., et al. 2014. Arch Biochem Biophys. 548: 74-85. PMID: 24632143
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- Identification of acetylcholinesterase inhibitors using homogenous cell-based assays in quantitative high-throughput screening platforms. | Li, S., et al. 2017. Biotechnol J. 12: PMID: 28294544
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- Hydrogen Peroxide-Based Fluorometric Assay for Real-Time Monitoring of SAM-Dependent Methyltransferases. | Akhtar, MK., et al. 2018. Front Bioeng Biotechnol. 6: 146. PMID: 30406092
- Optimization of Experimental Settings for the Assessment of Reactive Oxygen Species Production by Human Blood. | Soares, T., et al. 2019. Oxid Med Cell Longev. 2019: 7198484. PMID: 30733852
- Properties of a cryptic lysyl oxidase from haloarchaeon Haloterrigena turkmenica. | Pestov, NB., et al. 2019. PeerJ. 7: e6691. PMID: 30984480
- Peroxide-driven catalysis of the heme domain of A. radioresistens cytochrome P450 116B5 for sustainable aromatic rings oxidation and drug metabolites production. | Ciaramella, A., et al. 2020. N Biotechnol. 54: 71-79. PMID: 31473254
- Photooxidation-induced fluorescence amplification system for an ultra-sensitive enzyme-linked immunosorbent assay (ELISA). | Heo, Y., et al. 2021. Sci Rep. 11: 5831. PMID: 33712666
- A low-cost, enzyme-coupled fluorescent assay for rapid quantification of glycolysis rate of cells. | Cai, L., et al. 2022. Anal Bioanal Chem. 414: 1987-1997. PMID: 34984508
Activator of:
Horseradish Peroxidase.Substrate of:
Fluorogenic, and HRP and AP.Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ADHP, 25 mg | sc-291898 | 25 mg | $507.00 |