Adh1 CRISPR/Cas9 KO Plasmid (m): sc-419005
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Adh1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Adh1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Adh1 CRISPR/Cas9 KO Plasmid (m) | sc-419005 | 20 µg | $397.00 |
Overview
Adh1 encodes alcohol dehydrogenase 1, a cytosolic oxidoreductase that catalyzes NAD+/NADH-dependent interconversion of short-chain alcohols and aldehydes, contributing to cellular redox balance and intermediary metabolism. In mouse tissues, ADH1 activity supports alcohol and xenobiotic metabolism and interfaces with pathways controlling aldehyde detoxification, oxidative stress responses, and lipid/carbonyl homeostasis. Perturbation of alcohol/aldehyde handling is relevant to models of hepatic injury, inflammation, and metabolic dysfunction, where altered NADH/NAD+ ratios and reactive aldehyde accumulation can influence mitochondrial function and signaling. As a metabolic enzyme with broad substrate specificity, Adh1 is frequently examined in studies of detoxification capacity and tissue-specific metabolic adaptation.
Adh1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Adh1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Adh1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Adh1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Adh1 protein expression.
This CRISPR knockout system enables efficient generation of Adh1-deficient cell models for investigation of Adh1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Adh1 exon(s) critical for Adh1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Adh1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Adh1 CRISPR/Cas9 KO Plasmid (m) and Adh1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Adh1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Adh1 HDR Plasmid (m) and Adh1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Adh1 homology arms to support homology-directed repair at defined Adh1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.