Adenylate cyclase 5/AC5/ADCY5 Double Nickase Plasmid (h): sc-401391-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Adenylate cyclase 5/AC5/ADCY5 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Adenylate cyclase 5/AC5/ADCY5 Double Nickase Plasmid (h) and Adenylate cyclase 5/AC5/ADCY5 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ADCY5. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Adenylate cyclase 5/AC5/ADCY5 Double Nickase Plasmid (h) | sc-401391-NIC | 20 µg | $410.00 | |||
Adenylate cyclase 5/AC5/ADCY5 Double Nickase Plasmid (h2) | sc-401391-NIC-2 | 20 µg | $410.00 |
ADCY5 encodes adenylate cyclase 5 (AC5), a membrane-associated enzyme that converts ATP to cyclic AMP, shaping second-messenger signaling downstream of GPCRs. AC5 is prominently linked to Gαs/Gαi-regulated pathways that modulate PKA- and EPAC-dependent phosphorylation programs affecting neuronal excitability, synaptic plasticity, and metabolic signaling. Through control of cAMP dynamics, ADCY5 influences transcriptional responses, ion channel activity, and feedback regulation of receptor signaling. Genetic and functional perturbations of ADCY5 have been associated with neurodevelopmental and movement-related phenotypes, supporting its relevance for studying dysregulated cAMP signaling in human cellular models.
Adenylate cyclase 5/AC5/ADCY5 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ADCY5 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ADCY5. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ADCY5 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ADCY5-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.