Adenylate cyclase 3/AC3/ADCY3 Double Nickase Plasmid (h): sc-400407-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Adenylate cyclase 3/AC3/ADCY3 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Adenylate cyclase 3/AC3/ADCY3 Double Nickase Plasmid (h) and Adenylate cyclase 3/AC3/ADCY3 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ADCY3. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Adenylate cyclase 3/AC3/ADCY3 Double Nickase Plasmid (h) | sc-400407-NIC | 20 µg | $410.00 | |||
Adenylate cyclase 3/AC3/ADCY3 Double Nickase Plasmid (h2) | sc-400407-NIC-2 | 20 µg | $410.00 |
Human ADCY3 (adenylate cyclase 3, AC3) is a membrane-associated adenylyl cyclase that catalyzes conversion of ATP to cAMP downstream of GPCR signaling. By controlling cAMP/PKA-dependent phosphorylation programs, ADCY3 influences sensory signaling, cellular excitability, and transcriptional responses mediated by CREB and other cAMP-responsive effectors. AC3 activity contributes to compartmentalized second-messenger dynamics that shape metabolic and neuroendocrine processes, including hypothalamic regulation of energy balance. Genetic and functional studies have linked ADCY3 dysregulation or variants to altered cAMP signaling phenotypes and disease-relevant traits such as obesity and related metabolic dysfunction, supporting its use as a mechanistic node in GPCR–cAMP pathway research.
Adenylate cyclase 3/AC3/ADCY3 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ADCY3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ADCY3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ADCY3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ADCY3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.