Adenosine A2A-R (h): 293T Lysate: sc-127942

Adenosine A2A-R (h): 293T Lysate is derived from the 293T cell line, a human embryonic kidney cell line modified to express the SV40 large T antigen, enhancing its ability to produce high levels of recombinant proteins. This lysate is enriched with the human Adenosine A2A receptor (A2A-R), a G protein-coupled receptor (GPCR) that plays a pivotal role in mediating cellular responses to adenosine, an important signaling molecule. A2A-R is involved in various physiological processes, including neurotransmission, immune response modulation, and cardiovascular function. In research, Adenosine A2A-R (h): 293T Lysate is used to study the signaling mechanisms and regulatory functions of the A2A receptor. Researchers utilize this lysate in Western blotting to detect and quantify A2A-R expression levels under different experimental conditions, facilitating the study of receptor regulation and expression patterns. The lysate is also employed in co-immunoprecipitation assays to identify and investigate interactions between A2A-R and other cellular proteins, which helps explain the receptor's role in signaling pathways. Additionally, functional assays, such as cAMP accumulation assays, are performed using this lysate to measure the activation of A2A-R and its downstream signaling effects. These assays provide insights into how A2A-R activation influences cellular responses and signaling cascades. The use of Adenosine A2A-R (h): 293T Lysate enables detailed investigations into the receptor's molecular mechanisms and its role in various biological processes, all within a controlled experimental framework. The origin and quality of the 293T cell line are rigorously maintained to ensure reliable and reproducible results in basic research focused on GPCR signaling and function.