Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Adenosine A1-R Antibody (D-5) Alexa Fluor® 790: sc-514337 AF790. Direct near-infrared western blot analysis of Adenosine A1-R expression in MCF7 (A), K-562 (B) and U-2 OS (C) whole cell lysates. Blocked with UltraCruz® Blocking Reagent: sc-516214.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Adenosine A1-R CRISPR Activation Plasmid (h): sc-401241-ACT
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Adenosine A1-R CRISPR Activation Plasmid (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
- Adenosine A1-R CRISPR Activation Plasmid (h) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
- The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by Adenosine A1-R CRISPR Activation Plasmid (h) and Adenosine A1-R CRISPR Activation Plasmid (h2) target distinct regulatory regions upstream of the ADORA1 transcriptional start site. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Adenosine A1-R Antibody (D-5): sc-514337
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Adenosine A1-R CRISPR Activation Plasmid (h) | sc-401241-ACT | 20 µg | $397.00 | |||
Adenosine A1-R CRISPR Activation Plasmid (h2) | sc-401241-ACT-2 | 20 µg | $397.00 |