ADCK5 Double Nickase Plasmid (m): sc-435282-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ADCK5 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ADCK5 Double Nickase Plasmid (m) and ADCK5 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Adck5. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ADCK5 Double Nickase Plasmid (m) | sc-435282-NIC | 20 µg | $410.00 | |||
ADCK5 Double Nickase Plasmid (m2) | sc-435282-NIC-2 | 20 µg | $410.00 |
Adck5 encodes the atypical protein kinase ADCK5, a member of the aarF domain-containing kinase family implicated in mitochondrial regulation and cellular energy homeostasis. ADCK proteins are linked to coenzyme Q biology and oxidative phosphorylation, positioning ADCK5 within pathways that influence redox balance, ATP production, and mitochondrial quality control. Perturbation of these processes can alter reactive oxygen species signaling and metabolic adaptation, making Adck5 a relevant target for mechanistic studies of mitochondrial dysfunction and related stress-response phenotypes in mouse cell and tissue models.
ADCK5 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Adck5 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Adck5. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Adck5 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Adck5-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.