ADAR2 (h): 293T Lysate: sc-117039

ADAR2 (h): 293T Lysate is derived from the 293T cell line, a human embryonic kidney cell line known for its high transfection efficiency due to the expression of the SV40 large T antigen. This lysate is enriched with Adenosine Deaminase Acting on RNA 2 (ADAR2), an enzyme that plays a crucial role in RNA editing. ADAR2 catalyzes the deamination of adenosine to inosine in double-stranded RNA (dsRNA), which can result in alterations of RNA sequence and function, affecting gene expression and the diversity of the transcriptome. In research, ADAR2 (h): 293T Lysate is utilized to study the mechanisms of RNA editing and its regulatory impact on cellular processes. This lysate is particularly valuable in assays such as Western blotting to detect and quantify ADAR2 expression and assess the protein's levels under various experimental conditions. Researchers also employ this lysate in RNA immunoprecipitation (RIP) assays to identify RNA substrates that interact with ADAR2, helping to explain the specific RNA sequences and structures that are targeted by this enzyme. Furthermore, ADAR2 activity assays are conducted using this lysate to measure the enzymatic conversion of adenosine to inosine in RNA substrates, providing insights into the functional consequences of RNA editing. The use of ADAR2 (h): 293T Lysate allows for a detailed exploration of RNA editing mechanisms and their effects on gene regulation and transcriptome diversity. The origin and quality of the 293T cell line are rigorously controlled to ensure consistent and reproducible results in experimental research focused on understanding the molecular functions of ADAR2 and its role in cellular homeostasis.