ADAMTS-15 Antibody (5F3): sc-517041
- ADAMTS-15 Antibody (5F3) is a mouse monoclonal IgG2a κ ADAMTS-15 antibody provided at 100 µg/ml
- raised against amino acids 863-950 representing partial length ADAMTS-15 of human origin
- recommended for detection of ADAMTS-15 of human origin by WB, IP and ELISA
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for ADAMTS-15 Antibody (5F3). This work is in progress.
ADAMTS-15 Antibody (5F3) is a mouse monoclonal IgG2a kappa light chain antibody that detects ADAMTS-15 protein of human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-ADAMTS-15 antibody (5F3) is available as a non-conjugated format. ADAMTS (a disintegrin and metalloproteinase domain with Thrombospondin type-1 modules) family consists of zinc-dependent proteases that play crucial roles in various physiological and pathological processes, including tissue remodeling and disease progression such as arthritis and cancer. ADAMTS-15 is characterized by a unique structure, which includes an amino-terminal propeptide domain, a metalloproteinase domain, a disintegrin-like domain, and a carboxy-terminal region containing multiple Thrombospondin type-1 motifs. This structural arrangement enables enzymatic activity and interactions with other proteins, allowing ADAMTS-15 to cleave aggrecan, a key component of cartilage, thereby influencing extracellular matrix dynamics. ADAMTS-15 expression is primarily observed in fetal tissues, including lung, kidney, and liver, where ADAMTS-15 functions as a weak aggrecanase, particularly in fetal kidney and liver. Research has shown that low ADAMTS-15 expression in human breast carcinoma, combined with elevated ADAMTS-8 levels, correlates with increased risk of cancer recurrence and mortality, highlighting ADAMTS-15′s potential as a biomarker in cancer prognosis.
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ADAMTS-15 Antibody (5F3) References:
- ADAMTS: a novel family of extracellular matrix proteases. | Tang, BL. 2001. Int J Biochem Cell Biol. 33: 33-44. PMID: 11167130
- Cloning, expression analysis, and structural characterization of seven novel human ADAMTSs, a family of metalloproteinases with disintegrin and thrombospondin-1 domains. | Cal, S., et al. 2002. Gene. 283: 49-62. PMID: 11867212
- Characterization of ADAMTS-9 and ADAMTS-20 as a distinct ADAMTS subfamily related to Caenorhabditis elegans GON-1. | Somerville, RP., et al. 2003. J Biol Chem. 278: 9503-13. PMID: 12514189
- Identification and characterization of ADAMTS-20 defines a novel subfamily of metalloproteinases-disintegrins with multiple thrombospondin-1 repeats and a unique GON domain. | Llamazares, M., et al. 2003. J Biol Chem. 278: 13382-9. PMID: 12562771
- ADAMTS-9 is synergistically induced by interleukin-1beta and tumor necrosis factor alpha in OUMS-27 chondrosarcoma cells and in human chondrocytes. | Demircan, K., et al. 2005. Arthritis Rheum. 52: 1451-60. PMID: 15880812
- ADAMTS8 and ADAMTS15 expression predicts survival in human breast carcinoma. | Porter, S., et al. 2006. Int J Cancer. 118: 1241-7. PMID: 16152618
- Metalloproteinase and inhibitor expression profiling of resorbing cartilage reveals pro-collagenase activation as a critical step for collagenolysis. | Milner, JM., et al. 2006. Arthritis Res Ther. 8: R142. PMID: 16919164
- The consensus coding sequences of human breast and colorectal cancers. | Sjöblom, T., et al. 2006. Science. 314: 268-74. PMID: 16959974
- ADAMTS-5 deficiency does not block aggrecanolysis at preferred cleavage sites in the chondroitin sulfate-rich region of aggrecan. | East, CJ., et al. 2007. J Biol Chem. 282: 8632-40. PMID: 17255106
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ADAMTS-15 Antibody (5F3) | sc-517041 | 100 µg/ml | $322.00 |