ADAM4 Antibody (D-4): sc-390853
- ADAM4 Antibody (D-4) is a mouse monoclonal IgG1 κ ADAM4 antibody provided at 200 µg/ml
- raised against amino acids 577-636 mapping near the C-terminus of ADAM4 of mouse origin
- ADAM4 Antibody (D-4) is recommended for detection of ADAM4 of mouse and rat origin by WB, IP, IF and ELISA
- Anti-ADAM4 Antibody (D-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ADAM4 (D-4): sc-390853.
- m-IgG1 BP-HRP is the preferred secondary detection reagent for ADAM4 Antibody (D-4) for WB applications. This reagent is now offered in a bundle with ADAM4 Antibody (D-4) (see ordering information below).
ADAM4 Antibody (D-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects ADAM4 protein of mouse and rat origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ADAM4 Antibody (D-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ADAM4, a member of the disintegrin and metalloproteinase (ADAM) family, plays a crucial role in cellular processes such as proteolysis, adhesion, and signaling, which are vital for normal physiological functions. ADAM4 is predominantly expressed in the testis, where ADAM4 is involved in spermatogenesis and the fusion of sperm and egg cells, highlighting ADAM4′s importance in reproductive biology. ADAM4 is found at lower levels in various adult tissues, indicating potential roles beyond reproduction. ADAM4′s structure includes a disintegrin domain that facilitates interactions with other proteins, which is essential for function in cell adhesion and signaling pathways. Peptides targeting the disintegrin domain of ADAM4 have been shown not to disrupt the attachment of spermatogenic cells to Sertoli cell surfaces, suggesting a nuanced role in maintaining cellular interactions during spermatogenesis.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ADAM4 Antibody (D-4) References:
- Cloning and chromosomal mapping of mouse ADAM11, ADAM22 and ADAM23. | Sagane, K., et al. 1999. Gene. 236: 79-86. PMID: 10433968
- ADAM 23/MDC3, a human disintegrin that promotes cell adhesion via interaction with the alphavbeta3 integrin through an RGD-independent mechanism. | Cal, S., et al. 2000. Mol Biol Cell. 11: 1457-69. PMID: 10749942
- ADAM23, a possible tumor suppressor gene, is frequently silenced in gastric cancers by homozygous deletion or aberrant promoter hypermethylation. | Takada, H., et al. 2005. Oncogene. 24: 8051-60. PMID: 16103878
- Role of integrins, tetraspanins, and ADAM proteins during the development of apoptotic bodies by spermatogenic cells. | Kierszenbaum, AL., et al. 2006. Mol Reprod Dev. 73: 906-17. PMID: 16557522
- Methylation profile of genes CDKN2A (p14 and p16), DAPK1, CDH1, and ADAM23 in head and neck cancer. | Calmon, MF., et al. 2007. Cancer Genet Cytogenet. 173: 31-7. PMID: 17284367
- ADAM23 plays multiple roles in neuronal differentiation of P19 embryonal carcinoma cells. | Sun, Y., et al. 2007. Neurochem Res. 32: 1217-23. PMID: 17333391
- Chromosomal assignment of four testis-expressed mouse genes from a new family of transmembrane proteins (ADAMs) involved in cell-cell adhesion and fusion. | Cho, C., et al. 1996. Genomics. 34: 413-7. PMID: 8786143
- A role for the disintegrin domain of cyritestin, a sperm surface protein belonging to the ADAM family, in mouse sperm-egg plasma membrane adhesion and fusion. | Yuan, R., et al. 1997. J Cell Biol. 137: 105-12. PMID: 9105040
- Metalloproteinase-like, disintegrin-like, cysteine-rich proteins MDC2 and MDC3: novel human cellular disintegrins highly expressed in the brain. | Sagane, K., et al. 1998. Biochem J. 334 (Pt 1): 93-8. PMID: 9693107
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ADAM4 Antibody (D-4) | sc-390853 | 200 µg/ml | $316.00 | |||
ADAM4 Antibody (D-4): m-IgG1 BP-HRP Bundle | sc-531744 | 200 µg Ab; 20 µg BP | $354.00 | |||
ADAM4 Antibody (D-4) AC | sc-390853 AC | 500 µg/ml, 25% agarose | $416.00 | |||
ADAM4 Antibody (D-4) HRP | sc-390853 HRP | 200 µg/ml | $316.00 | |||
ADAM4 Antibody (D-4) FITC | sc-390853 FITC | 200 µg/ml | $330.00 | |||
ADAM4 Antibody (D-4) PE | sc-390853 PE | 200 µg/ml | $343.00 | |||
ADAM4 Antibody (D-4) Alexa Fluor® 488 | sc-390853 AF488 | 200 µg/ml | $357.00 | |||
ADAM4 Antibody (D-4) Alexa Fluor® 546 | sc-390853 AF546 | 200 µg/ml | $357.00 | |||
ADAM4 Antibody (D-4) Alexa Fluor® 594 | sc-390853 AF594 | 200 µg/ml | $357.00 | |||
ADAM4 Antibody (D-4) Alexa Fluor® 647 | sc-390853 AF647 | 200 µg/ml | $357.00 | |||
ADAM4 Antibody (D-4) Alexa Fluor® 680 | sc-390853 AF680 | 200 µg/ml | $357.00 | |||
ADAM4 Antibody (D-4) Alexa Fluor® 790 | sc-390853 AF790 | 200 µg/ml | $357.00 |