Anti-ADAM4 Antibody (D-4) is a mouse monoclonal IgG1 (kappa light chain) ADAM4 antibody provided at 200 µg/ml
raised against amino acids 577-636 mapping near the C-terminus of ADAM4 of mouse origin
Anti-ADAM4 Antibody (D-4) is recommended for detection of ADAM4 of mouse and rat origin by WB, IP, IF and ELISA
Anti-ADAM4 Antibody (D-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ADAM4 (D-4): sc-390853.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
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Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
ADAM4 Antibody (D-4) is a high quality monoclonal ADAM4 antibody (also designated ADAM4 antibody) suitable for the detection of the ADAM4 protein of mouse and rat origin. ADAM4 Antibody (D-4) is available as both the non-conjugated anti-ADAM4 antibody form, as well as multiple conjugated forms of anti-ADAM4 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. ADAMs (disintegrin and metalloproteinase domain), also known as MDCs (metalloproteinase, disintegrin, and cysteine-rich domain) or cellular disintegrins are a family of proteins that are ubiquitously expressed. They catalyze proteolysis, adhesion, fusion and intracellular signaling. ADAMs are membrane-anchored, glycosylated, Zn2+ dependent proteases, and there are over 30 different members in the family with many diverse functions. ADAM1-6 localize to the testis, are developmentally regulated, and are involved in spermatogenesis and sperm-egg binding and fusion. ADAM4 expression occurs postmeiotically in spermatids. In addition to its expression in the testis, ADAM4 is also found at low levels in all other adult tissues. Peptides targeting the disintegrin domain of ADAM4 do not disturb spermatogenic cell attachment to Sertoli cell surfaces.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
What is the recommended fixation method for immunofluorescence staining with ADAM4 (D-4): sc-390853 monoclonal antibody?
Asked by: cjMara
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here:
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