ADAM32 Antibody (A-5): sc-376738
- ADAM32 Antibody (A-5) is a mouse monoclonal IgG1 κ ADAM32 antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 531-680 mapping within an extracellular domain of ADAM32 of human origin
- ADAM32 Antibody (A-5) is recommended for detection of ADAM32 of human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-ADAM32 Antibody (A-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGκ BP-HRP is the preferred secondary detection reagent for ADAM32 Antibody (A-5) for WB and IHC(P) applications. This reagent is now offered in a bundle with ADAM32 Antibody (A-5) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
ADAM32 Antibody (A-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects ADAM32 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-ADAM32 antibody (A-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ADAM32, a member of the ADAM (disintegrin and metalloproteinase domain) family, is primarily expressed in the testis, particularly on the surface of sperm cells, where ADAM32 plays a crucial role in sperm-egg adhesion and fertilization processes. ADAM32 undergoes significant post-translational modifications, including glycosylation, which are essential for stability, activity, and interaction with other proteins. These modifications can influence ADAM32′s localization and function, making them vital for proper reproductive biology. ADAM32 precursor is synthesized in the testis and is processed during epididymal maturation, highlighting ADAM32′s importance in sperm development and function. Understanding ADAM32′s role and modifications can provide insights into male fertility and potential therapeutic targets for reproductive disorders.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ADAM32 Antibody (A-5) References:
- Cloning and chromosomal mapping of mouse ADAM11, ADAM22 and ADAM23. | Sagane, K., et al. 1999. Gene. 236: 79-86. PMID: 10433968
- ADAM 23/MDC3, a human disintegrin that promotes cell adhesion via interaction with the alphavbeta3 integrin through an RGD-independent mechanism. | Cal, S., et al. 2000. Mol Biol Cell. 11: 1457-69. PMID: 10749942
- Identification and characterization of ADAM32 with testis-predominant gene expression. | Choi, I., et al. 2003. Gene. 304: 151-62. PMID: 12568724
- Positive selection at reproductive ADAM genes with potential intercellular binding activity. | Glassey, B. and Civetta, A. 2004. Mol Biol Evol. 21: 851-9. PMID: 14963094
- ADAM23, a possible tumor suppressor gene, is frequently silenced in gastric cancers by homozygous deletion or aberrant promoter hypermethylation. | Takada, H., et al. 2005. Oncogene. 24: 8051-60. PMID: 16103878
- Role of integrins, tetraspanins, and ADAM proteins during the development of apoptotic bodies by spermatogenic cells. | Kierszenbaum, AL., et al. 2006. Mol Reprod Dev. 73: 906-17. PMID: 16557522
- Chromosomal assignment of four testis-expressed mouse genes from a new family of transmembrane proteins (ADAMs) involved in cell-cell adhesion and fusion. | Cho, C., et al. 1996. Genomics. 34: 413-7. PMID: 8786143
- A role for the disintegrin domain of cyritestin, a sperm surface protein belonging to the ADAM family, in mouse sperm-egg plasma membrane adhesion and fusion. | Yuan, R., et al. 1997. J Cell Biol. 137: 105-12. PMID: 9105040
- Metalloproteinase-like, disintegrin-like, cysteine-rich proteins MDC2 and MDC3: novel human cellular disintegrins highly expressed in the brain. | Sagane, K., et al. 1998. Biochem J. 334 (Pt 1): 93-8. PMID: 9693107
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ADAM32 Antibody (A-5) | sc-376738 | 200 µg/ml | $316.00 | |||
ADAM32 Antibody (A-5): m-IgGκ BP-HRP Bundle | sc-523074 | 200 µg Ab, 40 µg BP | $354.00 | |||
ADAM32 Antibody (A-5) AC | sc-376738 AC | 500 µg/ml, 25% agarose | $416.00 | |||
ADAM32 Antibody (A-5) HRP | sc-376738 HRP | 200 µg/ml | $316.00 | |||
ADAM32 Antibody (A-5) FITC | sc-376738 FITC | 200 µg/ml | $330.00 | |||
ADAM32 Antibody (A-5) PE | sc-376738 PE | 200 µg/ml | $343.00 | |||
ADAM32 Antibody (A-5) Alexa Fluor® 488 | sc-376738 AF488 | 200 µg/ml | $357.00 | |||
ADAM32 Antibody (A-5) Alexa Fluor® 546 | sc-376738 AF546 | 200 µg/ml | $357.00 | |||
ADAM32 Antibody (A-5) Alexa Fluor® 594 | sc-376738 AF594 | 200 µg/ml | $357.00 | |||
ADAM32 Antibody (A-5) Alexa Fluor® 647 | sc-376738 AF647 | 200 µg/ml | $357.00 | |||
ADAM32 Antibody (A-5) Alexa Fluor® 680 | sc-376738 AF680 | 200 µg/ml | $357.00 | |||
ADAM32 Antibody (A-5) Alexa Fluor® 790 | sc-376738 AF790 | 200 µg/ml | $357.00 |