ADAM28 Antibody (H-4): sc-514228
- ADAM28 Antibody (H-4) is a mouse monoclonal IgG3 κ ADAM28 antibody, cited in 3 publications, provided at 200 µg/ml
- raised against amino acids 143-307 mapping within an internal region of ADAM28 of mouse origin
- ADAM28 Antibody (H-4) is recommended for detection of ADAM28 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-ADAM28 Antibody (H-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for ADAM28 Antibody (H-4). This work is in progress.
ADAM28 Antibody (H-4) is a mouse monoclonal IgG3 kappa light chain antibody that detects ADAM28 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-ADAM28 antibody (H-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ADAM28, also known as MDC-L, is a member of the ADAM (a disintegrin and metalloprotease) family, which consists of over 30 membrane-anchored, glycosylated, zinc-dependent proteases that play crucial roles in cell-cell and cell-matrix interactions. ADAM28 is primarily located on the cell surface, where ADAM28 facilitates cell adhesion through interaction with integrin α4β1, a process vital for lymphocyte migration and function. ADAM28 is highly expressed in lymphocytes and the epididymis, indicating a significant role in immune response and reproductive processes. The gene encoding human ADAM28 is located on chromosome 8p12, and unique structural features, including a metalloprotease domain and a disintegrin domain, contribute to functional diversity and importance in various physiological processes, such as fertilization and modulation of neurogenic signaling pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ADAM28 Antibody (H-4) References:
- ADAM28 participates in the regulation of tooth development. | Zhao, Z., et al. 2006. Arch Oral Biol. 51: 996-1005. PMID: 16836973
- ADAM28 is a serological and histochemical marker for non-small-cell lung cancers. | Kuroda, H., et al. 2010. Int J Cancer. 127: 1844-56. PMID: 20112342
- ADAM28 manipulates proliferation, differentiation, and apoptosis of human dental pulp stem cells. | Zhao, Z., et al. 2011. J Endod. 37: 332-9. PMID: 21329817
- Effect of ADAM28 on carcinoma cell metastasis by cleavage of von Willebrand factor. | Mochizuki, S., et al. 2012. J Natl Cancer Inst. 104: 906-22. PMID: 22636800
- ADAM28 is expressed by epithelial cells in human normal tissues and protects from C1q-induced cell death. | Miyamae, Y., et al. 2016. FEBS J. 283: 1574-94. PMID: 26918856
- The Metalloproteinase ADAM28 Promotes Metabolic Dysfunction in Mice. | Herat, L., et al. 2017. Int J Mol Sci. 18: PMID: 28430139
- ADAM28 localizes to HLA-G+ trophoblasts and promotes column cell outgrowth. | De Luca, LC., et al. 2017. Placenta. 55: 71-80. PMID: 28623976
- ADAM28 promotes tumor growth and dissemination of acute myeloid leukemia through IGFBP-3 degradation and IGF-I-induced cell proliferation. | Zhang, JM., et al. 2019. Cancer Lett. 442: 193-201. PMID: 30429106
- ADAM28 from both endothelium and gastric cancer cleaves von Willebrand Factor to eliminate von Willebrand Factor-induced apoptosis of gastric cancer cells. | Yin, Q., et al. 2021. Eur J Pharmacol. 898: 173994. PMID: 33675784
- Role for the metalloproteinase ADAM28 in the control of airway inflammation, remodelling and responsiveness in asthma. | Bendavid, G., et al. 2022. Front Immunol. 13: 1067779. PMID: 36685493
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ADAM28 Antibody (H-4) | sc-514228 | 200 µg/ml | $322.00 | |||
ADAM28 Antibody (H-4) AC | sc-514228 AC | 500 µg/ml, 25% agarose | $424.00 | |||
ADAM28 Antibody (H-4) HRP | sc-514228 HRP | 200 µg/ml | $322.00 | |||
ADAM28 Antibody (H-4) FITC | sc-514228 FITC | 200 µg/ml | $336.00 | |||
ADAM28 Antibody (H-4) PE | sc-514228 PE | 200 µg/ml | $349.00 | |||
ADAM28 Antibody (H-4) Alexa Fluor® 488 | sc-514228 AF488 | 200 µg/ml | $364.00 | |||
ADAM28 Antibody (H-4) Alexa Fluor® 546 | sc-514228 AF546 | 200 µg/ml | $364.00 | |||
ADAM28 Antibody (H-4) Alexa Fluor® 594 | sc-514228 AF594 | 200 µg/ml | $364.00 | |||
ADAM28 Antibody (H-4) Alexa Fluor® 647 | sc-514228 AF647 | 200 µg/ml | $364.00 | |||
ADAM28 Antibody (H-4) Alexa Fluor® 680 | sc-514228 AF680 | 200 µg/ml | $364.00 | |||
ADAM28 Antibody (H-4) Alexa Fluor® 790 | sc-514228 AF790 | 200 µg/ml | $364.00 |