ADAM12 Antibody (1G3) recommended for detection of ADAM12 of mouse, rat and human origin by WB, IP and ELISA

ADAM12 Antibody (1G3): sc-293225

ADAM12 Antibody (1G3) is rated 4.0 out of 5 by 2.
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Datasheets

    • Anti-ADAM12 Antibody (1G3) is a mouse monoclonal IgG3 (kappa light chain) ADAM12 antibody provided at 100 µg/ml
    • raised against a partial recombinant protein mapping within amino acids 208-304 of ADAM12 of human origin
    • recommended for detection of ADAM12 of mouse, rat and human origin by WB, IP and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ADAM12 (1G3): sc-293225.
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Gibt es zu dem Antikörper eine Liste von sicheren Positiv -und Negativkontrollen in spezifischen Geweben? Es wäre sehr hilfreich um den AK korrekt zu validieren.

Asked by: CarlottaKE
Vielen Dank für Ihre Frage. Leider gibt es nur wenige Daten zu der Expression von ADAM12. Ein Gewebe für das starke Expression von ADAM12 beschrieben ist, ist humanes Plazentagewebe. Der Antikörper wurde in unseren Labors mit ADAM12-transfizierten HEK293T-Zelllysaten etabliert. Untransfizierte HEK293T Zellen exprimieren das Protein nicht und wurden als Negativkontrolle verwendet. Bitte kontaktieren Sie unseren technichschen Service wenn Sie weitere Fragen haben sollten. "Is there a list of positive and negative control tissues for this antibody?" Thank you for your question. Unfortunately, there is only little data regarding the expression of ADAM12. Robust expression of ADAM12 has been described for human placental tissue. We have established this antibody using ADAM12-transfected HEK293T whole cell lysates. Untransfected HEK293T cells do not express the protein and were used as negative controls. Please contact our technical service if you have further questions.
Answered by: Technical Support Europe
Date published: 2019-01-15

For Western Blot, is it recommended to use denatured or non-denatured conditions with ADAM12 (1G3): sc-293225 antibody?

Asked by: AbPolly
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-03-27
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Rated 4 out of 5 by from 有主要检测的条带 之前申请了试用装,用来做westernblot能检测出好几个条带。这也跟这个蛋白有几个不同的形式有关吧。推荐使用。
Date published: 2017-07-05
Rated 4 out of 5 by from 条带单一,非常好 之前订购了试用装,用作WB,条带明显,符合试验检测要求. 条件1:1000稀释,用作结直肠癌细胞系蛋白的检测
Date published: 2017-03-17
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