ACYP1 Antibody (2-RE16): sc-134246

ACYP1 Antibody (2-RE16) is a mouse monoclonal IgG2b kappa light chain antibody that detects ACYP1 protein of human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). ACYP1 (2-RE16) antibody is available as a non-conjugated format. Acylphosphatase, encoded by ACYP1, plays a crucial role in cellular metabolism by catalyzing the hydrolysis of carboxyl-phosphate bonds in acylphosphates, which is essential for regulating various biochemical pathways. ACYP1′s importance extends beyond enzymatic function; ACYP1 is primarily located in the cytosol, where ACYP1 participates in acylphosphate dephosphorylation, thereby influencing lipid metabolism and energy production. ACYP1′s presence in the cytosol allows for rapid response to changes in cellular energy demands, making ACYP1 vital for maintaining cellular homeostasis. Additionally, two isoenzymes, ACYP1 and ACYP2, share 60% homology, highlighting this enzyme family′s evolutionary significance. While both isoenzymes exhibit similar substrate specificity, ACYP1 shows higher catalytic activity compared to ACYP2, which has been extensively studied as a model for protein misfolding and aggregation. Understanding ACYP1′s functional dynamics remains essential for elucidating ACYP1′s role in metabolic disorders and potential therapeutic targets.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

ACYP1 Antibody (2-RE16) References:

1. Kinetic partitioning of protein folding and aggregation.  |  Chiti, F., et al. 2002. Nat Struct Biol. 9: 137-43. PMID: 11799398
2. Studies of the aggregation of mutant proteins in vitro provide insights into the genetics of amyloid diseases.  |  Chiti, F., et al. 2002. Proc Natl Acad Sci U S A. 99 Suppl 4: 16419-26. PMID: 12374855
3. A nucleophilic catalysis step is involved in the hydrolysis of aryl phosphate monoesters by human CT acylphosphatase.  |  Paoli, P., et al. 2003. J Biol Chem. 278: 194-9. PMID: 12409302
4. ACYP1 gene possesses two alternative splicing forms that induce apoptosis.  |  Degl'Innocenti, D., et al. 2004. IUBMB Life. 56: 29-33. PMID: 14992377
5. Effects of acylphosphatase on the activity of erythrocyte membrane Ca2+ pump.  |  Nassi, P., et al. 1991. J Biol Chem. 266: 10867-71. PMID: 1645713
6. Immunoaffinity purification and immunoassay determination of human erythrocyte acylphosphatase.  |  Degl'Innocenti, D., et al. 1990. Biotechnol Appl Biochem. 12: 450-9. PMID: 2169259
7. A new acylphosphatase isoenzyme from human erythrocytes: purification, characterization, and primary structure.  |  Liguri, G., et al. 1986. Biochemistry. 25: 8089-94. PMID: 3026468
8. Cloning and expression of the cDNA coding for the erythrocyte isoenzyme of human acylphosphatase.  |  Fiaschi, T., et al. 1995. FEBS Lett. 367: 145-8. PMID: 7796909
9. Assignment of the human erythrocyte acylphosphatase gene (ACYP1) to chromosome band 14q24.3.  |  Fiaschi, T., et al. 1998. Cytogenet Cell Genet. 81: 235-6. PMID: 9730610