Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ACTR-IIB Double Nickase Plasmid (h): sc-401414-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ACTR-IIB Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ACTR-IIB Double Nickase Plasmid (h) and ACTR-IIB Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ACVR2B. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ACTR-IIB Antibody (G-7): sc-376593
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ACTR-IIB Double Nickase Plasmid (h) | sc-401414-NIC | 20 µg | $410.00 | |||
ACTR-IIB Double Nickase Plasmid (h2) | sc-401414-NIC-2 | 20 µg | $410.00 |
ACVR2B encodes activin receptor type IIB (ACTR-IIB), a transmembrane serine/threonine kinase receptor in the TGF-β superfamily that binds activins, myostatin (GDF8), and related ligands. Upon ligand engagement, ACTR-IIB forms complexes with type I receptors to activate SMAD2/3 signaling and modulate transcriptional programs controlling proliferation, differentiation, and tissue homeostasis. This axis is a central regulator of skeletal muscle and metabolic biology and also influences reproductive and inflammatory signaling via activin pathways. Dysregulated ACVR2B/ACTR-IIB signaling has been associated with altered muscle mass phenotypes, fibrosis-related processes, and tumor biology contexts, supporting its use as a mechanistic node in pathway-focused studies.
ACTR-IIB Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ACVR2B locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ACVR2B. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ACVR2B function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ACVR2B-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.