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Acrylamide (CAS 79-06-1)

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Alternate Names:
Acrylic acid amide; Propenamide
Application:
Acrylamide is a highly toxic, but commonly used matrix for electrophoretic separation of nucleic acids and proteins
CAS Number:
79-06-1
Purity:
≥98%
Molecular Weight:
71.08
Molecular Formula:
C3H5NO
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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Acrylamide is a used matrix for electrophoretic separation of nucleic acids and proteins. Acrylamide forms linear polymers and bis-Acrylamide introduces cross-links between polyacrylamide chains. By mixing the appropriate ratio of Acrylamide to bis-Acrylamide, the optimal pore size can be obtained for the protein being resolved. To avoid the toxicity of dry acrylamide, ready-to-use acrylamide solutions are available. Acrylamide Solution 40% (sc-3721) and bis-Acrylamide 2% (sc-3719) solutions offer convenience and eliminate the risks associated with the handling of acrylamide powder. Polymerization agents, TEMED (sc-29111) and APS (Ammonium persulfate, sc-202946) are also available. For denaturing gels, SDS (20% Solution) (sc-24950) is available.


Acrylamide (CAS 79-06-1) References

  1. Two-dimensional gel electrophoresis in proteomics: old, old fashioned, but it still climbs up the mountains.  |  Rabilloud, T. 2002. Proteomics. 2: 3-10. PMID: 11788986
  2. Two-Dimensional Gel Electrophoresis.  |  Raymond, S. and Aurell, B. 1962. Science. 138: 152-3. PMID: 17818402
  3. Studies of acrylamide level in coffee and coffee substitutes: influence of raw material and manufacturing conditions.  |  Mojska, H. and Gielecińska, I. 2013. Rocz Panstw Zakl Hig. 64: 173-81. PMID: 24325083
  4. Acrylamide Induced Toxicity and the Propensity of Phytochemicals in Amelioration: A Review.  |  Kunnel, SG., et al. 2019. Cent Nerv Syst Agents Med Chem. 19: 100-113. PMID: 30734688
  5. Acrylamide in coffee: formation and possible mitigation strategies - a review.  |  Schouten, MA., et al. 2020. Crit Rev Food Sci Nutr. 60: 3807-3821. PMID: 31905027
  6. Developmental and Neurotoxicity of Acrylamide to Zebrafish.  |  Park, JS., et al. 2021. Int J Mol Sci. 22: PMID: 33805345
  7. The Protective Agents Used against Acrylamide Toxicity: An In Vitro Cell Culture Study-Based Review.  |  Kacar, S. and Sahinturk, V. 2021. Cell J. 23: 367-381. PMID: 34455711
  8. Acrylamide inhibits long-term potentiation and learning involving microglia and pro-inflammatory signaling.  |  Izumi, Y., et al. 2022. Sci Rep. 12: 12429. PMID: 35858988
  9. Acrylamide; a neurotoxin in popcorns: a systematic review and meta-analysis.  |  Abedini, A., et al. 2022. Rev Environ Health.. PMID: 35960600
  10. A short history of electrophoretic methods.  |  Vesterberg, O. 1993. Electrophoresis. 14: 1243-9. PMID: 8137783
  11. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis of human parotid salivary proteins: comparison of dansylation, coomassie blue R-250 and silver detection methods.  |  Beeley, JA., et al. 1996. Electrophoresis. 17: 505-6. PMID: 8740168
  12. Effect of protein application mode and acrylamide concentration on the resolution of protein spots separated by two-dimensional gel electrophoresis.  |  Langen, H., et al. 1997. Electrophoresis. 18: 2085-90. PMID: 9420174
  13. Extraction of membrane proteins by differential solubilization for separation using two-dimensional gel electrophoresis.  |  Molloy, MP., et al. 1998. Electrophoresis. 19: 837-44. PMID: 9629924

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Acrylamide, 100 g

sc-214492
100 g
$36.00

Acrylamide, 500 g

sc-214492A
500 g
$112.00

Acrylamide, 1 kg

sc-214492B
1 kg
$173.00