Date published: 2025-9-5
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Acrp30 Antibody (31) - Western Blotting - Image 281412
Acrp30 抗体 (31): sc-136131. 人血浆中 Acrp30 的 Western 印迹分析 (A) 和人脂肪组织提取物中 Acrp30 的表达 (B).
Acrp30 抗体 (31): sc-136131. 人血浆中 Acrp30 的 Western 印迹分析 (A) 和人脂肪组织提取物中 Acrp30 的表达 (B).

Acrp30 抗体 (31): sc-136131

4.5(6)
寫評論提問

说明书
  • Acrp30 抗体 31 是小鼠单克隆 IgG2a κ，Acrp30抗体, 在10篇文献中引用，规格为200 µg/ml
  • 免疫human物种的代表全长Acrp30的氨基酸1-247
  • 推荐用于 和 human 来源的Acrp30 WB 和 IP检测
  • 还可偶联琼脂糖 用于IP; 偶联HRP 用于WB, IHC(P) 和ELISA
  • 关于如何获取Acrp30 (31): sc-136131的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和m-IgGκ BP-HRP是Acrp30 Antibody (31) 适用于 WB 应用。 的首选辅助检测试剂，这些试剂现与Acrp30 Antibody (31) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

Acrp30抗体（31）是一种IgG2a κ小鼠单克隆Acrp30抗体（也称为ADIPO抗体、脂联素抗体、含C1Q和胶原结构域的抗体、GBP28抗体、脂肪细胞补体相关30 kDa蛋白抗体、ACDC抗体、APM1抗体或ADPN抗体），可通过WB和IP检测人源Acrp30蛋白。Acrp30抗体（脂肪细胞补体相关蛋白或AdipoQ）是一种仅在脂肪细胞中合成的分泌蛋白，在脂肪细胞分化过程中诱导的mRNA超过100倍。Acrp30的转录后修饰产生多种不同分子量的寡聚形式，包括单体、二聚体、三聚体、六聚体和聚合物。Acrp30是一种丰富的血清蛋白，仅由脂肪细胞分泌，与能量稳态和肥胖有关。由于人类和小鼠肥胖病例中Acrp30的失调以及与TNFα的强烈结构相似性，Acrp30被怀疑是全身能量稳态的调节因子。此外，Acrp30受调节的胞吐作用似乎需要磷脂酰肌醇-3-激酶活性，因为胰岛素刺激的Acrp30分泌被该酶的药物抑制剂阻断。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Acrp30 抗体 (31) 参考文献:

  1. 由内源性 ACRP30 和 GLUT4 定义的 3T3-L1 脂肪细胞中胰岛素刺激的两个外泌区。  |  Bogan, JS. and Lodish, HF. 1999. J Cell Biol. 146: 609-20. PMID: 10444069
  2. 编码脂肪细胞特异性分泌蛋白 Acrp30 的小鼠基因的染色体定域, 表达模式和启动子分析。  |  Das, K., et al. 2001. Biochem Biophys Res Commun. 280: 1120-9. PMID: 11162643
  3. 30-kDa脂肪细胞补体相关蛋白的蛋白水解裂解产物可增加肌肉中的脂肪酸氧化，并导致小鼠体重减轻。  |  Fruebis, J., et al. 2001. Proc Natl Acad Sci U S A. 98: 2005-10. PMID: 11172066
  4. 离子霉素, 二丁烯酰环AMP和肿瘤坏死因子-α对分化的原代人类前脂肪细胞中apM1的细胞内数量和分泌的影响。  |  Kappes, A. and Löffler, G. 2000. Horm Metab Res. 32: 548-54. PMID: 11246823
  5. 脂细胞补体相关蛋白30 kDa（Acrp30）依赖寡聚状态激活NF-κB信号通路。  |  Tsao, TS., et al. 2002. J Biol Chem. 277: 29359-62. PMID: 12087086
  6. 脂联素/Acrp30在体内具有抗糖尿病和抗动脉粥样硬化的双重作用。  |  Yamauchi, T., et al. 2003. Curr Drug Targets Immune Endocr Metabol Disord. 3: 243-54. PMID: 14683455
  7. 要形成高分子量的低聚物复合物，就必须对脂肪连通素胶原结构域内的四个保守赖氨酸残基进行翻译后修饰。  |  Wang, Y., et al. 2006. J Biol Chem. 281: 16391-400. PMID: 16621799
  8. Acrp30通过在侵略性SPEC-2子宫内膜癌细胞中激活AMPK来下调JAK/STAT3通路，从而抑制瘦素诱导的转移。  |  Wu, X., et al. 2012. Oncol Rep. 27: 1488-96. PMID: 22327423
  9. 腺病毒载体rAAV2/1-Acrp30对动脉粥样硬化Goto-Kakizaki大鼠的葡萄糖、脂质代谢和主动脉超微结构的影响。  |  Li, QX., et al. 2012. Natl Med J India. 25: 5-9. PMID: 22680312
  10. 一种类似于 C1q 的新型血清蛋白，只在脂肪细胞中产生。  |  Scherer, PE., et al. 1995. J Biol Chem. 270: 26746-9. PMID: 7592907
  11. 补体-1q 家族蛋白的晶体结构表明它与肿瘤坏死因子存在进化联系。  |  Shapiro, L. and Scherer, PE. 1998. Curr Biol. 8: 335-8. PMID: 9512423

订购信息

产品名称产品编号规格价格数量收藏夹

Acrp30 抗体 (31)

sc-136131
200 µg/ml
$316.00

Acrp30 (31): m-IgG Fc BP-HRP 套装

sc-528800
200 µg Ab; 10 µg BP
$354.00

Acrp30 (31): m-IgGκ BP-HRP 套装

sc-521330
200 µg Ab, 40 µg BP
$354.00

Acrp30 抗体 (31) AC

sc-136131 AC
500 µg/ml, 25% agarose
$416.00

Acrp30 抗体 (31) HRP

sc-136131 HRP
200 µg/ml
$316.00

Hi, I wanted to know whether this antibody can detect the all the isoforms of the adiponectin in WB (Native non-reduced and non-heated SDS-PAGE)

Asked by: Karthikka
Thank you for your question. This antibody can potentially detect different iso forms of the Acrp30 target though we have only seen expression around ~30 kDa. There is also a possibility of a dimer forming under reducing conditions. We recommend running your WB under reducing and denaturing conditions. If you have any further questions, please contact Technical Service.
Answered by: Technical Service 3
Date published: 2020-11-20

Could this antibody detects the HMW adiponectin in western blot technique?

Asked by: Karthikka
Thank you for your question. Acrp30 Antibody (31): sc-136131 is recommended for detection of Acrp30 of human origin by WB and IP.
Answered by: Tech Support Europe
Date published: 2020-11-19

what is the type of secondary antibody used?

Asked by: lommy
Thank you for your inquiry. An anti-mouse IgG will be ok. For more detailed information about this question, please call us toll-free at (800) 457-3801 option 2, e-mail <scbt@scbt.com>, or use the live chat on our website.
Answered by: Technical Support 15
Date published: 2018-02-01

I am using Acrp30 (31): sc-136131 antibody for immunoprecipitation, how much antibody should I use?

Asked by: Dr Ninau Qelp
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from clean antibody for human acrp30This antibody is so clean that I showed the whole blot in the picture. We used it for human adipocyte whole protein lysate at 1:3000 in 4% BSA.
Date published: 2019-11-01
Rated 5 out of 5 by from Works perfectly in human adipose tissue.Antibody tested in western blot with 3 ug of human adipose tissue.
Date published: 2018-05-06
Rated 4 out of 5 by from This antibody is working fineIt gives clear bands at the right positioning, in dilution 1:200
Date published: 2017-05-30
Rated 5 out of 5 by from Robust antibodyVery strong Western blot band observed with Acrp30 Antibody (31). Highly recommended!
Date published: 2017-01-26
Rated 4 out of 5 by from Strong bands seen in WB using 3T3Strong bands seen in WB using 3T3-L1, HeLa and Raji whole cell lysates. -SCBT QC
Date published: 2015-06-29
Rated 4 out of 5 by from Robust band seen in WB using protein purifiedRobust band seen in WB using protein purified from human plasma. -SCBT QC
Date published: 2014-12-17
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Acrp30 抗体 (31) is rated 4.5 out of 5 by 6.
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