Acrosin Double Nickase Plasmid (h): sc-402292-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Acrosin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Acrosin Double Nickase Plasmid (h) and Acrosin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ACR. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Acrosin Double Nickase Plasmid (h) | sc-402292-NIC | 20 µg | $410.00 | |||
Acrosin Double Nickase Plasmid (h2) | sc-402292-NIC-2 | 20 µg | $410.00 |
ACR encodes acrosin, a trypsin-like serine protease stored as proacrosin within the acrosome of human spermatozoa and activated during fertilization. Acrosin contributes to acrosomal matrix dispersal and localized proteolysis of glycoprotein substrates at the zona pellucida, supporting sperm–egg interactions and penetration. Its function intersects with regulated protease activation, acrosome reaction dynamics, and extracellular matrix remodeling at the gamete interface. Altered acrosin activity or acrosomal proteostasis has been associated with impaired fertilization potential and is investigated in studies of male reproductive biology and infertility-related phenotypes.
Acrosin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ACR locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ACR. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ACR function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ACR-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.