ACP2 Antibody (B-7): sc-390667
- ACP2 Antibody (B-7) is a mouse monoclonal IgG2a κ ACP2 antibody provided at 200 µg/ml
- raised against amino acids 151-256 mapping within an internal region of ACP2 of human origin
- ACP2 Antibody (B-7) is recommended for detection of ACP2 of human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-ACP2 Antibody (B-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ACP2 Antibody (B-7) for WB and IHC(P) applications. These reagents are now offered in bundles with ACP2 Antibody (B-7) (see ordering information below).
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ACP2 Antibody (B-7) is a mouse monoclonal IgG2a kappa light chain antibody that detects ACP2 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). ACP2 Antibody (B-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ACP2, or acid phosphatase 2, is a 423 amino acid enzyme belonging to the histidine acid phosphatase family, primarily localized in the lysosomal compartment of cells. The enzyme plays a crucial role in the hydrolysis of orthophosphoric monoesters into alcohols and phosphates, which is essential for various metabolic processes, including the degradation of cellular components and the recycling of phosphate groups. The optimal enzymatic activity of ACP2 occurs at an acidic pH, characteristic of the lysosomal environment, highlighting its importance in cellular homeostasis and nutrient recycling. Deficiencies in the gene encoding ACP2 can lead to acid phosphatase deficiency, a severe condition that manifests with symptoms such as terminal bleeding, opisthotonos, hypotonia, lethargy, intermittent vomiting, and can result in death during early infancy.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ACP2 Antibody (B-7) References:
- Mutation in the gene encoding lysosomal acid phosphatase (Acp2) causes cerebellum and skin malformation in mouse. | Mannan, AU., et al. 2004. Neurogenetics. 5: 229-38. PMID: 15503243
- Structure of the human lysosomal acid phosphatase gene. | Geier, C., et al. 1989. Eur J Biochem. 183: 611-6. PMID: 2776754
- Human lysosomal acid phosphatase: cloning, expression and chromosomal assignment. | Pohlmann, R., et al. 1988. EMBO J. 7: 2343-50. PMID: 3191910
- Cathepsin G, acid phosphatase, and alpha 1-proteinase inhibitor messenger RNA levels in keratoconus corneas. | Whitelock, RB., et al. 1997. Invest Ophthalmol Vis Sci. 38: 529-34. PMID: 9040486
- Genetic polymorphism of rabbit (Oryctolagus cuniculus) tissue acid phosphatases (ACP2 and ACP3). | Branco, M. and Ferrand, N. 1998. Comp Biochem Physiol B Biochem Mol Biol. 120: 405-9. PMID: 9787802
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ACP2 Antibody (B-7) | sc-390667 | 200 µg/ml | $316.00 | |||
ACP2 Antibody (B-7): m-IgG Fc BP-HRP Bundle | sc-530218 | 200 µg Ab; 10 µg BP | $354.00 | |||
ACP2 Antibody (B-7): m-IgGκ BP-HRP Bundle | sc-523627 | 200 µg Ab, 40 µg BP | $354.00 | |||
ACP2 Antibody (B-7) AC | sc-390667 AC | 500 µg/ml, 25% agarose | $416.00 | |||
ACP2 Antibody (B-7) HRP | sc-390667 HRP | 200 µg/ml | $316.00 | |||
ACP2 Antibody (B-7) FITC | sc-390667 FITC | 200 µg/ml | $330.00 | |||
ACP2 Antibody (B-7) PE | sc-390667 PE | 200 µg/ml | $343.00 | |||
ACP2 Antibody (B-7) Alexa Fluor® 488 | sc-390667 AF488 | 200 µg/ml | $357.00 | |||
ACP2 Antibody (B-7) Alexa Fluor® 546 | sc-390667 AF546 | 200 µg/ml | $357.00 | |||
ACP2 Antibody (B-7) Alexa Fluor® 594 | sc-390667 AF594 | 200 µg/ml | $357.00 | |||
ACP2 Antibody (B-7) Alexa Fluor® 647 | sc-390667 AF647 | 200 µg/ml | $357.00 | |||
ACP2 Antibody (B-7) Alexa Fluor® 680 | sc-390667 AF680 | 200 µg/ml | $357.00 | |||
ACP2 Antibody (B-7) Alexa Fluor® 790 | sc-390667 AF790 | 200 µg/ml | $357.00 |