AChE Double Nickase Plasmid (h): sc-401091-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- AChE Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- AChE Double Nickase Plasmid (h) and AChE Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ACHE. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: AChE Antibody (A-11): sc-373901
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
AChE Double Nickase Plasmid (h) | sc-401091-NIC | 20 µg | $410.00 | |||
AChE Double Nickase Plasmid (h2) | sc-401091-NIC-2 | 20 µg | $410.00 |
Human ACHE encodes acetylcholinesterase (AChE), a serine hydrolase that rapidly terminates cholinergic neurotransmission by hydrolyzing acetylcholine at synapses and neuromuscular junctions. Beyond neurotransmitter clearance, AChE contributes to cholinergic signaling homeostasis, synaptic plasticity, and cell–cell communication, with isoform- and localization-dependent functions at the cell surface and extracellular matrix. Dysregulated ACHE expression or activity is linked to altered neuronal excitability and has been associated with neurodegenerative and neurodevelopmental phenotypes, as well as neuromuscular dysfunction. In experimental systems, ACHE perturbation is used to interrogate cholinergic pathway regulation, synaptic organization, and enzyme structure–function relationships.
AChE Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ACHE locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ACHE. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ACHE function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ACHE-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.