Acetylthiocholine iodide (CAS 1866-15-5)

Acetylthiocholine iodide is a synthetic compound, functioning as a cholinergic substrate, primarily utilized in various biochemical assays aimed at investigating the activity of enzymes such as acetylcholinesterase (AChE) and butyrylcholinesterase (BChE). These enzymes play critical roles in the hydrolysis of choline esters, and acetylthiocholine iodide serves as a specific substrate for the quantification of their enzymatic activity. Upon hydrolysis by AChE or BChE, acetylthiocholine iodide yields thiocholine, which can be detected and quantified through colorimetric or electrochemical methods, allowing for the assessment of enzyme activity. This compound′s utility extends to the study of enzyme kinetics, enzyme inhibition, and the screening of inhibitors that could influence cholinergic functions, contributing significantly to the understanding of enzyme mechanisms at a molecular level. Its role in research has been pivotal in elucidating the dynamics of neurotransmitter regulation and enzyme functionality, providing insights into the biochemical pathways that underlie cholinergic signaling.