ACES (CAS 7365-82-4)

ACES, or N-(2-Acetamido)-2-aminoethanesulfonic acid, serves as a vital buffering agent in various scientific research applications, particularly in biochemical and molecular biology studies. The mechanism of action of ACES lies in its ability to maintain stable pH conditions due to its pKa value close to physiological pH. This unique property makes ACES an ideal choice for buffering solutions used in enzymatic assays, protein purification, and nucleic acid hybridization experiments, where maintaining precise pH levels is critical for optimal reaction conditions. In addition to its role as a buffering agent, ACES has been extensively utilized in electrophoresis techniques for DNA and protein analysis. Its buffering capacity helps maintain stable pH gradients during gel electrophoresis procedures, ensuring accurate separation and analysis of biomolecules. Moreover, ACES finds applications in neuroscience research, where it is incorporated into artificial cerebrospinal fluid (aCSF) formulations to mimic the ionic composition and pH of natural cerebrospinal fluid for in vitro studies on neuronal function and synaptic transmission. Furthermore, ACES is commonly used in cell culture media formulations to maintain physiological pH levels, supporting cell growth and viability in laboratory settings. Overall, ACES′s compatibility with biological systems and its ability to regulate pH make it an indispensable tool in various scientific research endeavors, facilitating advancements in biochemistry, molecular biology, neuroscience, and cell biology.