ACE2 Double Nickase Plasmid (h): sc-401131-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ACE2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ACE2 Double Nickase Plasmid (h) and ACE2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ACE2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ACE2 Antibody (E-11): sc-390851
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ACE2 Double Nickase Plasmid (h) | sc-401131-NIC | 20 µg | $410.00 | |||
ACE2 Double Nickase Plasmid (h2) | sc-401131-NIC-2 | 20 µg | $410.00 |
Angiotensin-converting enzyme 2 (ACE2) is a zinc-dependent carboxypeptidase that counterbalances the renin–angiotensin system by converting angiotensin II to angiotensin-(1–7), thereby shifting signaling from vasoconstrictive, pro-inflammatory axes toward MAS receptor–linked pathways. Human ACE2 is expressed on epithelial and endothelial surfaces and contributes to regulation of vascular tone, tissue remodeling, oxidative stress responses, and inflammatory signaling. In addition to its enzymatic role, ACE2 influences receptor trafficking and membrane protease networks that shape cell-surface proteostasis. Dysregulated ACE2 expression or activity has been associated with cardiovascular and renal pathology, metabolic inflammation, and epithelial barrier dysfunction, making it a key target for mechanistic studies of host response and tissue homeostasis.
ACE2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ACE2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ACE2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ACE2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ACE2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.