ACE Antibody (B-6): sc-374198

ACE Antibody (B-6) is a mouse monoclonal IgG2b kappa light chain antibody designed to detect angiotensin-converting enzyme (ACE), also known as CD143 or Angiotensin Converting Enzyme 1. Anti-ACE antibody (B-6) targets a precise epitope mapping between amino acids 1279-1306 at the C-terminus of human ACE, enabling reliable detection of both somatic and testis isoforms of ACE from mouse, rat, and human origins through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ACE monoclonal antibody (B-6) is an essential tool for researchers studying the structural biology of ACE, a zinc metallopeptidase composed of two homologous domains, each containing an active site crucial for enzymatic function in the renin-angiotensin system. The C-terminal domain, recognized by ACE (B-6) antibody, is vital for the dimeric structure of ACE, which is necessary for effective substrate binding and catalytic activity, thereby playing a key role in regulating blood pressure and maintaining electrolyte balance. The structural integrity of ACE, maintained by its two-domain configuration, is essential for converting Angiotensin I to the potent vasopressor hormone Angiotensin II and inactivating bradykinin, a vasodepressor peptide. Additionally, ACE exists in two main isoforms: the pulmonary ACE (ACEP), a glycoprotein expressed in vascular endothelial cells of organs such as the lung, liver, adrenal cortex, pancreas, kidney, and spleen, and the testicular ACE (ACET), which is exclusively expressed in adult testis by developing sperm cells, specifically late pachytene spermatocytes. Understanding the structural aspects of ACE is critical for investigating its role in cardiovascular diseases, hypertension, and related disorders, making ACE (B-6) monoclonal antibody an indispensable tool in biomedical research.

The preferred ACE antibody is ACE Antibody (2E2): sc-23908.