ACE Antibody (2E2): sc-23908

ACE Antibody (2E2) is a mouse monoclonal IgG1 kappa light chain antibody designed to target angiotensin-converting enzyme (ACE), also known as CD143, DCP1, or Angiotensin Converting Enzyme 1. ACE monoclonal antibody (2E2) effectively detects ACE proteins from mouse, rat, and human origins through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). Available in both non-conjugated and multiple conjugated forms such as agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates, anti-ACE antibody (2E2) provides versatile options for diverse research needs. Angiotensin-converting enzyme (ACE) is a crucial carboxy-terminal dipeptidyl exo-peptidase involved in the renin-angiotensin system, where ACE converts Angiotensin I into the potent vasopressor hormone Angiotensin II, playing a vital role in regulating blood pressure and electrolyte balance. Additionally, ACE inactivates bradykinin, a vasodepressor peptide, thereby contributing to fluid and electrolyte homeostasis. There are two primary isoforms of ACE: pulmonary ACE (ACEP), a glycoprotein expressed in vascular endothelial cells of the lung, liver, adrenal cortex, pancreas, kidney, and spleen, and testicular ACE (ACET), which is exclusively expressed in adult testis by developing sperm cells, specifically late pachytene spermatocytes. Understanding ACE function is essential for researching cardiovascular diseases, hypertension, and related disorders, making anti-ACE antibody (2E2) an indispensable tool in biomedical research. Furthermore, while ACE is ubiquitously expressed throughout the vasculature, ACE2 has a more restricted expression pattern, being limited to cardiac, renal, and testicular cells, highlighting the importance of precise detection provided by ACE (2E2) antibody.

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ACE Antibody (2E2) References:

1. A novel angiotensin-converting enzyme-related carboxypeptidase (ACE2) converts angiotensin I to angiotensin 1-9.  |  Donoghue, M., et al. 2000. Circ Res. 87: E1-9. PMID: 10969042
2. Use of alternative polyadenylation sites for tissue-specific transcription of two angiotensin-converting enzyme mRNAs.  |  Thekkumkara, TJ., et al. 1992. Nucleic Acids Res. 20: 683-7. PMID: 1311831
3. Angiotensin-converting enzyme: vascular endothelial localization.  |  Caldwell, PR., et al. 1976. Science. 191: 1050-1. PMID: 175444
4. Angiotensin-converting enzyme and the regulation of vasoactive peptides.  |  Soffer, RL. 1976. Annu Rev Biochem. 45: 73-94. PMID: 183603
5. The mRNAs encoding the two angiotensin-converting isozymes are transcribed from the same gene by a tissue-specific choice of alternative transcription initiation sites.  |  Kumar, RS., et al. 1991. J Biol Chem. 266: 3854-62. PMID: 1847388
6. An enzyme in microsomal fraction of kidney that inactivates bradykinin.  |  Erdos, EG. and Yang, HY. 1967. Life Sci. 6: 569-74. PMID: 4962200
7. Molecular and catalytic properties of rabbit testicular dipeptidyl carboxypeptidase.  |  El-Dorry, HA., et al. 1982. J Biol Chem. 257: 14128-33. PMID: 6754723
8. Regulated expression of testis angiotensin-converting enzyme during spermatogenesis in mice.  |  Langford, KG., et al. 1993. Biol Reprod. 48: 1210-8. PMID: 8391328

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ACE Antibody (2E2) has 37 citations in a variety of scientific publications.

Primary antibodies like Anti-ACE Antibody (2E2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ACE Antibody (2E2) Specifications

Alternate Product Name: ACE

Product Clone Name: 2E2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: ACE

Epitope Species: human

Product Reactivity/Detection: ACE

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 37 citations in various scientific publications

Available Conjugates/Forms for ACE Antibody (2E2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin