Date published: 2026-2-2

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Ac-YVAD-pNA (CAS 149231-66-3)

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Alternate Names:
Caspase-1 Substrate IV, Colorimetric
Application:
Ac-YVAD-pNA is a substrate for caspase-1 and caspase-4; cleavage is monitored colorimetrically at 405nm
CAS Number:
149231-66-3
Purity:
≥97%
Molecular Weight:
628.63
Molecular Formula:
C29H36N6O10
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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Ac-YVAD-pNA, a synthetic peptide, has gained significant popularity in scientific research owing to its remarkable ability to efficiently and rapidly inhibit caspases. Caspases, a family of cysteine proteases for apoptosis or programmed cell death, can be effectively targeted by Ac-YVAD-pNA. This peptide has emerged as an indispensable tool for studying apoptosis and investigating the roles of caspases in various diseases and disorders. Ac-YVAD-pNA exerts its inhibitory effects by binding to the active site of caspases, effectively preventing their substrate cleavage and impeding the initiation of apoptosis. Importantly, this inhibition is reversible and can be counteracted by the introduction of a reducing agent, such as dithiothreitol (DTT). Moreover, Ac-YVAD-pNA possesses the remarkable ability to simultaneously inhibit multiple caspases, rendering it an invaluable tool for examining the impact of caspase inhibition on intricate cell signaling pathways.


Ac-YVAD-pNA (CAS 149231-66-3) References

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  3. Wogonin and fisetin induction of apoptosis through activation of caspase 3 cascade and alternative expression of p21 protein in hepatocellular carcinoma cells SK-HEP-1.  |  Chen, YC., et al. 2002. Arch Toxicol. 76: 351-9. PMID: 12107653
  4. Differential apoptosis-inducing effect of quercetin and its glycosides in human promyeloleukemic HL-60 cells by alternative activation of the caspase 3 cascade.  |  Shen, SC., et al. 2003. J Cell Biochem. 89: 1044-55. PMID: 12874837
  5. A novel heterodimeric cysteine protease is required for interleukin-1 beta processing in monocytes.  |  Thornberry, NA., et al. 1992. Nature. 356: 768-74. PMID: 1574116
  6. Some commonly used caspase substrates and inhibitors lack the specificity required to monitor individual caspase activity.  |  Pereira, NA. and Song, Z. 2008. Biochem Biophys Res Commun. 377: 873-7. PMID: 18976637
  7. Staphylococcus aureus induces apoptosis in primary bovine mammary epithelial cells through Fas-FADD death receptor-linked caspase-8 signaling.  |  Hu, Q., et al. 2014. DNA Cell Biol. 33: 388-97. PMID: 24564258
  8. Rutin modulates ASC expression in NLRP3 inflammasome: a study in alcohol and cerulein-induced rat model of pancreatitis.  |  Aruna, R., et al. 2014. Mol Cell Biochem. 396: 269-80. PMID: 25060908
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  10. A novel effector caspase (Caspase-3/7-1) involved in the regulation of immune homeostasis in Chinese mitten crab Eriocheir sinensis.  |  Qu, C., et al. 2018. Fish Shellfish Immunol. 83: 76-83. PMID: 30195917
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Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Ac-YVAD-pNA, 1 mg

sc-311283
1 mg
$47.00

Ac-YVAD-pNA, 5 mg

sc-311283A
5 mg
$114.00