Molecular structure of Ac-YVAD-AFC, CAS Number: 219137-85-6
Ac-YVAD-AFC (CAS 219137-85-6)
See product citations (3)
Alternate Names:
Ac-Tyr-Val-Ala-Asp-AFC; Caspase-1 substrate (fluorogenic), Caspase-4 substrate (fluorogenic)
Application:
Ac-YVAD-AFC is a fluorogenic substrate for caspase-1 and caspase-4
CAS Number:
219137-85-6
Purity:
≥97%
Molecular Weight:
719.67
Molecular Formula:
C33H36F3N5O10
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.
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SDS & Certificate of Analysis
Ac-YVAD-AFC is a fluorogenic substrate for caspase-1 and caspase-4. Ac-YVAD-AFC functions by specifically targeting caspase-1, a enzyme involved in the inflammatory response. Caspase-1 plays a significant role in cleaving pro-inflammatory cytokines, including interleukin-1β and interleukin-18. By binding to the active site of caspase-1, Ac-YVAD-AFC hinders the cleavage of pro-inflammatory cytokines, thereby mitigating inflammation. Its effects extend to diverse biochemical and physiological aspects, encompassing the inhibition of pro-inflammatory cytokine production like interleukin-1β and interleukin-18, as well as the suppression of other inflammatory mediators such as nitric oxide and prostaglandin E2. Moreover, Ac-YVAD-AFC diminishes the activation of NF-κB, a transcription factor pivotal in the inflammatory process.
Ac-YVAD-AFC (CAS 219137-85-6) References
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- Rengyolone inhibits inducible nitric oxide synthase expression and nitric oxide production by down-regulation of NF-kappaB and p38 MAP kinase activity in LPS-stimulated RAW 264.7 cells. | Kim, JH., et al. 2006. Biochem Pharmacol. 71: 1198-205. PMID: 16457781
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- Measuring NLR Oligomerization I: Size Exclusion Chromatography, Co-immunoprecipitation, and Cross-Linking. | Khare, S., et al. 2016. Methods Mol Biol. 1417: 131-43. PMID: 27221486
- Mitochondrial function is required for extracellular ATP-induced NLRP3 inflammasome activation. | Sadatomi, D., et al. 2017. J Biochem. 161: 503-512. PMID: 28096454
- Substance P and IL-33 administered together stimulate a marked secretion of IL-1β from human mast cells, inhibited by methoxyluteolin. | Taracanova, A., et al. 2018. Proc Natl Acad Sci U S A. 115: E9381-E9390. PMID: 30232261
- Clathrin-Mediated Endocytosis Delivers Proteolytically Active Phytaspases Into Plant Cells. | Trusova, SV., et al. 2019. Front Plant Sci. 10: 873. PMID: 31379892
- Calcium Pyrophosphate And Monosodium Urate Activate The NLRP3 Inflammasome Within Bladder Urothelium Via Reactive Oxygen Species And TXNIP. | Harper, SN., et al. 2019. Res Rep Urol. 11: 319-325. PMID: 31819864
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Substrate of:
caspase-1, caspase-4, Enzyme, and Fluorogenic.Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ac-YVAD-AFC, 5 mg | sc-311282 | 5 mg | $163.00 | |||
Ac-YVAD-AFC, 10 mg | sc-311282A | 10 mg | $316.00 |