Ac-YEVD-AMC
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The structure of Ac-YEVD-AMC stands for N-acetyl-tyrosine-glutamic acid-valine-aspartic acid-7-amino-4-methylcoumarin. It is a small peptide consisting of four amino acids (YEVD) attached to a fluorogenic molecule, 7-amino-4-methylcoumarin (AMC), through an acetyl group (Ac). Caspase substrate: Ac-YEVD-AMC is specifically designed to be cleaved by caspase enzymes. Caspases are a family of protease enzymes involved in programmed cell death (apoptosis) and inflammation. They play roles in various physiological and pathological processes. Ac-YEVD-AMC is a fluorogenic substrate, which means it does not fluoresce until it is cleaved by caspase enzymes. Upon cleavage, the peptide bond between the valine and AMC is broken, releasing the highly fluorescent AMC molecule. The increase in fluorescence can be measured using a spectrofluorometer or fluorescence plate reader, providing a quantitative measure of caspase activity.
Ac-YEVD-AMC References
- Shiga toxin suppresses noncanonical inflammasome responses to cytosolic LPS. | Havira, MS., et al. 2020. Sci Immunol. 5: PMID: 33246946
- Substrate specificities of caspase family proteases. | Talanian, RV., et al. 1997. J Biol Chem. 272: 9677-82. PMID: 9092497
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ac-YEVD-AMC, 5 mg | sc-300160 | 5 mg | $208.00 | |||
Ac-YEVD-AMC, 50 mg | sc-300160A | 50 mg | $1800.00 |