MW: 688.7
A fluorogenic substrate for caspase-1 and caspase-4.


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Synonym: Caspase-1/Caspase-4 Substrate II, Fluorogenic
Application: A fluorogenic substrate for caspase-1 and caspase-4
Molecular Weight: 688.7
Molecular Formula: C35H40N6O9
* Refer to Certificate of Analysis for lot specific data (including water content).
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Ac-WVAD-AMC is a fluorogenic substrate for caspase-1 (kcat/Km = 2.41 x 105 M-1s-1) and caspase-4. Ac-WVAD-AMC exhibits ~2 fold higher affinity towards caspase-1 than for caspase-4.


1. D. Lee, et al. 1999. Bioorg. Med. Chem. Lett. 9 (12), 1667-1672. PMID: 10397497

2. T.A. Rano, et al. 1997. Chem. Biol. 4(2), 149-155. PMID: 9190289

Appearance :
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Storage :
Store at -20° C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

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Is there a protocol for this product (target concentration, pH range for fluorophore, excitation and emission wavelengths, etc.)? Also, is this fluorophore quenched by the peptide and fluorescent only when cleaved?

Asked by: Ray_B
sc-300159 is a fluorogenic substrate for caspase-4 and caspase-1 (kcat/Km = 2.41 x 105 M-1s-1). Ac-WVAD-AMC exhibits approximately 2 fold greater affinity towards caspase-1 than for caspase-4. Fluorescence is seen after cleavage. In assay the AMC liberated from the fluorogenic substrate was measured in a spectrofluorometer with an excitation wavelength of 380 nm and emission wavelength range of 430-460 nm. Reconstitute sc-300159 before use in 1 ml DMSO for a concentration of 1mg/mg in DMSO. Store the reconstituted substrate at -20°C for up to 1-2 months and avoid repeated freeze-thaw cycles. REFERENCES: D. Lee, et al., Bioorg. Med. Chem. Lett. 9, 1667 (1999) T.A. Rano, et al., Chem. Biol. 4, 149 (1997)
Answered by: Technical Support
Date published: 2019-01-26
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