Molecular structure of Ac-VEID-AFC
Ac-VEID-AFC
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Alternate Names:
Ac-Val-Glu-Ile-Asp-AFC
Application:
Ac-VEID-AFC is a fluorogenic substrate for caspase-6
Molecular Weight:
727.68
Molecular Formula:
C32H40F3N5O11
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.
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SDS & Certificate of Analysis
Ac-VEID-AFC is a fluorogenic substrate for caspase-6 (Km=30muM Kcat/Km=168000 M-1sec-1) and related cysteine proteases. The sequence is based on the lamin A site of cleavage. The activity is measured by the release of free fluorescent 7-amino-4-trifluoromethylcoumarin (AFC), which excites at 395-400 nm and emits at 495-505 nm.
Ac-VEID-AFC References
- Pro-caspase-3 overexpression sensitises ovarian cancer cells to proteasome inhibitors. | Tenev, T., et al. 2001. Cell Death Differ. 8: 256-64. PMID: 11319608
- Isolation of AmphiCASP-3/7, an ancestral caspase from amphioxus (Branchiostoma floridae). Evolutionary considerations for vertebrate caspases. | Bayascas, JR., et al. 2002. Cell Death Differ. 9: 1078-89. PMID: 12232796
- Proteasomal degradation of caspase-6 in 17beta-estradiol-treated neurons. | Tounekti, O., et al. 2004. J Neurochem. 89: 561-8. PMID: 15086513
- Caspase-1 activation of caspase-6 in human apoptotic neurons. | Guo, H., et al. 2006. Cell Death Differ. 13: 285-92. PMID: 16123779
- Bis(2-hydroxybenzylidene)acetone, a potent inducer of the phase 2 response, causes apoptosis in mouse leukemia cells through a p53-independent, caspase-mediated pathway. | Dinkova-Kostova, AT., et al. 2007. Cancer Lett. 245: 341-9. PMID: 16517063
- Dinitrosyl-iron triggers apoptosis in Jurkat cells despite overexpression of Bcl-2. | Kleschyov, AL., et al. 2006. Free Radic Biol Med. 40: 1340-8. PMID: 16631524
- A quantitative method for the specific assessment of caspase-6 activity in cell culture. | Ehrnhoefer, DE., et al. 2011. PLoS One. 6: e27680. PMID: 22140457
- Tacrolimus-Induced Apoptosis is Mediated by Endoplasmic Reticulum-derived Calcium-dependent Caspases-3,-12 in Jurkat Cells. | Chung, YW., et al. 2018. Transplant Proc. 50: 1172-1177. PMID: 29731088
- Identification of Allosteric Inhibitors against Active Caspase-6. | Tubeleviciute-Aydin, A., et al. 2019. Sci Rep. 9: 5504. PMID: 30940883
- Arabidopsis thaliana phytaspase: identification and peculiar properties. | Chichkova, NV., et al. 2018. Funct Plant Biol. 45: 171-179. PMID: 32291031
- Rare CASP6N73T variant associated with hippocampal volume exhibits decreased proteolytic activity, synaptic transmission defect, and neurodegeneration. | Zhou, L., et al. 2021. Sci Rep. 11: 12695. PMID: 34135352
- The Ced-3/interleukin 1beta converting enzyme-like homolog Mch6 and the lamin-cleaving enzyme Mch2alpha are substrates for the apoptotic mediator CPP32. | Srinivasula, SM., et al. 1996. J Biol Chem. 271: 27099-106. PMID: 8900201
- Substrate specificities of caspase family proteases. | Talanian, RV., et al. 1997. J Biol Chem. 272: 9677-82. PMID: 9092497
- A combinatorial approach defines specificities of members of the caspase family and granzyme B. Functional relationships established for key mediators of apoptosis. | Thornberry, NA., et al. 1997. J Biol Chem. 272: 17907-11. PMID: 9218414
Substrate of:
caspase-6, Enzyme, and Fluorogenic.Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ac-VEID-AFC, 5 mg | sc-311280 | 5 mg | $256.00 | |||
Ac-VEID-AFC, 10 mg | sc-311280A | 10 mg | $456.00 |