Molecular structure of Ac-DEVD-AFC, CAS Number: 201608-14-2
Ac-DEVD-AFC (CAS 201608-14-2)
See product citations (13)
Alternate Names:
Ac-DEVD-AFC is also known as Caspase-3 Substrate (Fluorogenic).
Application:
Ac-DEVD-AFC is a fluorogenic substrate for caspase-3 and related cysteine proteases, used as an indicator for cellular caspase-3 effects.
CAS Number:
201608-14-2
Purity:
≥90%
Molecular Weight:
729.61
Molecular Formula:
C30H34F3N5O13
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.
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SDS & Certificate of Analysis
Ac-DEVD-AFC is a fluorogenic substrate for caspase-3 (CPP32) and related cysteine proteases, which emits a blue to green shift in fluorescence. It also acts as a substrate for granzyme B-processed caspase-6, caspase-7, caspase-8, and caspase-10. It is known that Ac-DEVD-AFC has a greater Stokes′ shift than Ac-DEVD-AMC once it has been cleaved. Since caspase-3 is an effector caspase, it is involved in the cleavage of many substrates which promote apoptosis. This indicator excites light at 400 nm and emits light at 505 nm. In addition, Ac-DEVD-AFC is modeled after Asp216 on caspase-3, the PARP cleavage site. Ac-DEVD-AFC is an essential indicator used to monitor the cellular effects of caspase-3.
Ac-DEVD-AFC (CAS 201608-14-2) References
- Influence of gangliosides on the IL-2- and IL-4-dependent cell proliferation. | Molotkovskaya, IM., et al. 2002. Neurochem Res. 27: 761-70. PMID: 12374211
- Caspase-mediated cleavage of the feline calicivirus capsid protein. | Al-Molawi, N., et al. 2003. J Gen Virol. 84: 1237-1244. PMID: 12692289
- Molecular mechanisms of apoptosis induced by Scorpio water extract in human hepatoma HepG2 cells. | Kwon, KB., et al. 2005. World J Gastroenterol. 11: 943-7. PMID: 15742393
- Gentamicin-induced apoptosis in LLC-PK1 cells: involvement of lysosomes and mitochondria. | Servais, H., et al. 2005. Toxicol Appl Pharmacol. 206: 321-33. PMID: 16039943
- Caspase activation by anticancer drugs: the caspase storm. | Tao, Z., et al. 2007. Mol Pharm. 4: 583-95. PMID: 17439154
- Influence of cytochrome c on apoptosis induced by Anagrapha (Syngrapha) falcifera multiple nuclear polyhedrosis virus (AfMNPV) in insect Spodoptera litura cells. | Liu, L., et al. 2007. Cell Biol Int. 31: 996-1001. PMID: 17478109
- Dose-dependent effects of the caspase inhibitor Q-VD-OPh on different apoptosis-related processes. | Kuželová, K., et al. 2011. J Cell Biochem. 112: 3334-42. PMID: 21751237
- Cloning, Escherichia coli expression, purification, characterization, and enzyme assay of the ribosomal protein S4 from wheat seedlings (Triticum vulgare). | Yadaiah, M., et al. 2012. Protein Expr Purif. 81: 55-62. PMID: 21945701
- Involvement of Bim in Photofrin-mediated photodynamically induced apoptosis. | Wang, X., et al. 2015. Cell Physiol Biochem. 35: 1527-36. PMID: 25791936
- Analysis of the minimal specificity of caspase-2 and identification of Ac-VDTTD-AFC as a caspase-2-selective peptide substrate. | Kitevska, T., et al. 2014. Biosci Rep. 34: PMID: 27919034
- Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis. | Nicholson, DW., et al. 1995. Nature. 376: 37-43. PMID: 7596430
- Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE. | Lazebnik, YA., et al. 1994. Nature. 371: 346-7. PMID: 8090205
- [Electric stimulation of the human auditory system with transtympanic electrodes]. | Bochenek, Z., et al. 1977. Otolaryngol Pol. 31: 225-8. PMID: 896209
- Caspase-7 is activated during lovastatin-induced apoptosis of the prostate cancer cell line LNCaP. | Marcelli, M., et al. 1998. Cancer Res. 58: 76-83. PMID: 9426061
- Different subcellular distribution of caspase-3 and caspase-7 following Fas-induced apoptosis in mouse liver. | Chandler, JM., et al. 1998. J Biol Chem. 273: 10815-8. PMID: 9556551
Substrate of:
caspase-10, caspase-3, caspase-6, caspase-7, caspase-8, Enzyme, and Fluorogenic.Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ac-DEVD-AFC, 1 mg | sc-311274 | 1 mg | $65.00 | |||
Ac-DEVD-AFC, 5 mg | sc-311274A | 5 mg | $200.00 |