ABTB1 Double Nickase Plasmid (h): sc-408539-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ABTB1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ABTB1 Double Nickase Plasmid (h) and ABTB1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ABTB1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ABTB1 Double Nickase Plasmid (h) | sc-408539-NIC | 20 µg | $410.00 | |||
ABTB1 Double Nickase Plasmid (h2) | sc-408539-NIC-2 | 20 µg | $410.00 |
ABTB1 (ankyrin repeat and BTB/POZ domain containing 1) encodes a cytosolic adaptor-like protein characterized by BTB/POZ and ankyrin repeat motifs that support protein–protein interactions and assembly of multiprotein complexes. ABTB1 has been linked to regulation of cellular proliferation and differentiation programs, with reported connections to ubiquitin-dependent quality control and cytoskeletal organization. Through these interaction networks, ABTB1 is studied in pathways that influence cell-cycle progression, stress responses, and maintenance of tissue homeostasis. Altered ABTB1 expression has been observed across multiple cancer-related datasets, making it a useful target for mechanistic studies of tumor-associated signaling and cellular fitness.
ABTB1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ABTB1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ABTB1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ABTB1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ABTB1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.