ABHD2 Double Nickase Plasmid (h): sc-411559-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ABHD2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ABHD2 Double Nickase Plasmid (h) and ABHD2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ABHD2. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ABHD2 Double Nickase Plasmid (h) | sc-411559-NIC | 20 µg | $410.00 |
ABHD2 (abhydrolase domain containing 2) encodes a human serine hydrolase associated with membrane compartments where it contributes to lipid metabolism and lipid-derived signaling. As a member of the α/β-hydrolase fold enzyme family, ABHD2 activity intersects with pathways regulating bioactive lipids, membrane composition, and downstream second-messenger responses that influence cell signaling and ion channel regulation. ABHD2 expression and enzymatic function have been investigated in the context of reproductive biology and broader metabolic and signaling phenotypes where dysregulated lipid handling can contribute to disease-associated cellular states. These features make ABHD2 a useful target for studies linking lipid enzymology to signaling networks and phenotype modulation in human cell models.
ABHD2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ABHD2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ABHD2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ABHD2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ABHD2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.