ABCG2 (h2): 293T Lysate: sc-172393

ABCG2 (h2): 293T Lysate is derived from the 293T cell line, a human embryonic kidney cell line engineered to express the SV40 large T antigen, which significantly enhances its transfection efficiency and protein expression capacity. This lysate is enriched with the ATP-binding cassette sub-family G member 2 (ABCG2), a crucial membrane protein involved in the transport of various molecules across extracellular and intracellular membranes. ABCG2, also known as the breast cancer resistance protein (BCRP), plays a significant role in cellular processes related to multidrug resistance, stem cell maintenance, and xenobiotic transport. In research, ABCG2 (h2): 293T Lysate is extensively used to study the mechanisms of drug transport and resistance. Researchers utilize this lysate in Western blotting to detect and quantify the expression levels of ABCG2, providing insights into its regulation under different experimental conditions. It is also employed in functional assays to assess the transport activity of ABCG2, including substrate specificity and inhibitor effects, which are critical for understanding its role in cellular detoxification and protection against xenobiotics. Additionally, the lysate is used in co-immunoprecipitation experiments to identify proteins that interact with ABCG2, elucidating the protein networks and signaling pathways associated with its function. The use of ABCG2 (h2): 293T Lysate enables detailed investigations into the biological roles and regulatory mechanisms of ABCG2, contributing to our understanding of its impact on cellular homeostasis and response to environmental challenges. The origin and quality of the 293T cell line are meticulously controlled, ensuring consistent and reproducible results in basic scientific research focused on membrane transport proteins and their physiological functions.