ABCG2 Double Nickase Plasmid (h): sc-416737-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ABCG2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ABCG2 Double Nickase Plasmid (h) and ABCG2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ABCG2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ABCG2 Antibody (B-1): sc-377176
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ABCG2 Double Nickase Plasmid (h) | sc-416737-NIC | 20 µg | $410.00 | |||
ABCG2 Double Nickase Plasmid (h2) | sc-416737-NIC-2 | 20 µg | $410.00 |
ABCG2 (also known as BCRP) encodes an ATP-binding cassette efflux transporter that limits intracellular accumulation of diverse xenobiotics and endogenous metabolites. Localized to the plasma membrane in barrier and stem-like cell populations, ABCG2 contributes to epithelial transport, cellular detoxification, and protection against oxidative and chemical stress through ATP-dependent substrate export. Its activity intersects with pharmacokinetic handling and stress-response programs by modulating intracellular exposure to small molecules and porphyrin-related metabolites. Dysregulated ABCG2 expression or function is frequently studied in the context of multidrug resistance phenotypes, tissue barrier biology, and tumor heterogeneity, as well as inherited variants impacting transporter activity.
ABCG2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ABCG2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ABCG2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ABCG2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ABCG2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.